FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells

Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the "S2" cell line lags behind more common insect cell...

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Bibliographic Details
Published in:	Frontiers in bioengineering and biotechnology Vol. 10; p. 871933
Main Authors:	Coker, Jesse A, Katis, Vittorio L, Fairhead, Michael, Schwenzer, Anja, Clemmensen, Stine B, Frandsen, Bent U, de Jongh, Willem A, Gileadi, Opher, Burgess-Brown, Nicola A, Marsden, Brian D, Midwood, Kim S, Yue, Wyatt W
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 05-05-2022
Subjects:	Bioengineering and Biotechnology glycoproteins insect cells recombinant proteins S2 cells secreted proteins structural biology recombinant proteins secreted proteins glycoproteins structural biology insect cells S2 cells
Online Access:	Get full text
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Summary:	Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the "S2" cell line lags behind more common insect cell lines such as Sf9 or High-Five™. Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represents a bottleneck, particularly for biochemical and biophysical laboratories. Therefore, we developed FAS2FURIOUS, a simple and rapid S2 expression pipeline built upon an existing low-throughput commercial platform. FAS2FURIOUS is comparable in effort to simple systems and allows users to clone and test up to 46 constructs in just 2 weeks. Given the ability of S2 cells to express challenging targets, including receptor ectodomains, secreted glycoproteins, and viral antigens, FAS2FURIOUS represents an attractive orthogonal approach for protein expression in eukaryotic cells.
Bibliography:	Reviewed by: Feng Wang, Southwest University, China This article was submitted to Synthetic Biology, a section of the journal Frontiers in Bioengineering and Biotechnology Kathrin Göritzer, St George’s University of London, United Kingdom Edited by: Jose Ruben Morones-Ramirez, Autonomous University of Nuevo León, Mexico
ISSN:	2296-4185 2296-4185
DOI:	10.3389/fbioe.2022.871933