Isolation and expeditious morphological, biochemical and kinetic characterization of propolis-tolerant ruminal bacteria
It was aimed in this work to evaluate bacterial strains tolerant to products based on propolis (LLOS) through the isolation, morphological and biochemical characterization techniques in diets with roughage:concentrate ratio 100:0 and 50:50. For roughage diets, the products LLOSC1 and LLOSB3 were eva...
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| Published in: | Revista brasileira de zootecnia Vol. 39; no. 9; pp. 2048 - 2054 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Zootecnia
01-09-2010
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | It was aimed in this work to evaluate bacterial strains tolerant to products based on propolis (LLOS) through the isolation, morphological and biochemical characterization techniques in diets with roughage:concentrate ratio 100:0 and 50:50. For roughage diets, the products LLOSC1 and LLOSB3 were evaluated, and for 50:50% diets, the products LLOSC1, LLOSD1, LLOSA2, and LLOSC3, which differed in alcoholic concentrations (1, 2 and 3) and propolis (A, B, C and D) concentrations. The ruminal liquid was anaerobically incubated at 39°C for 6 days in medium containing LLOS. After isolation, the strains were submitted to Gram staining and the bacterial growth was monitored by photospectrometer. It was evaluated the strain growth in the presence of the following subtracts: arabinose, cellulose, glucose, cellobiose, xylose, fructose, and lactose. In roughage diets, strains tolerant to LLOSC1 and LLOSB3 were similar to carbohydrates degradation, except lactose in which LLOSC1 was superior to strains tolerant to LLOSB3. For diets with 50:50 roughage:concentrate ratio, the products LLOSC3 and LLOSA2 stood out because they selected the highest number of strains able to degrade most of the tested carbohydrates. The results suggest that tolerance to propolis is higher in Gram-positive strains with several growth metabolic levels.
Objetivou-se avaliar cepas bacterianas tolerantes a produtos à base de própolis pelas técnicas de isolamento, caracterização morfológica e bioquímica, em dietas com relação volumoso: concentrado de 100:0 e 50:50. Para dietas volumosas foram avaliados os produtos LLOSC1 e LLOSB3 e, para dietas 50:50% os produtos LLOSC1, LLOSD1, LLOSA2 e LLOSC3, diferentes quanto aos teores alcoólicos (1, 2 e 3) e as concentrações de própolis (A, B, C e D). O líquido ruminal foi incubado anaerobiamente a 39°C durante 6 dias em meio contendo LLOS. Após o isolamento, as cepas foram submetidas à coloração de Gram e o crescimento bacteriano foi monitorado por espectrofotômetro. Foi avaliado o crescimento das cepas na presença dos substratos: arabinose, celulose, glicose, celobiose, xilose, frutose e lactose. Em dietas volumosas as cepas tolerantes a LLOSC1 e LLOSB3 foram semelhantes na degradação dos carboidratos, exceto lactose no qual LLOSC1 foi superior às cepas tolerantes a LLOSB3. Para dieta 50:50 de volumoso:concentrado, destacaram-se os produtos LLOSC3 e LLOSA2, que selecionaram maior número de cepas capazes de degradar a maioria dos carboidratos testados. Os resultados sugerem que a tolerância à própolis foi maior nas cepas Gram-positivas, com níveis metabólicos de crescimento diversos. |
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| Bibliography: | http://www.scielo.br/scielo.php?pid=S1516-35982010000900025&script=sci_abstract&tlng=pt |
| ISSN: | 1516-3598 1806-9290 1806-9290 1516-3598 |
| DOI: | 10.1590/S1516-35982010000900025 |