Frequency of Interferon-γ-Producing T Cells Specific for Trypanosoma cruzi Inversely Correlates with Disease Severity in Chronic Human Chagas Disease

This study sought to quantify CD8+ T cell responses to Trypanosoma cruzi and to identify potential links between these responses and the severity of disease in humans. In the majority of patients with Chagas disease, staining with class I major histocompatibility complex tetramers and analysis of in...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 189; no. 5; pp. 909 - 918
Main Authors:	Laucella, Susana A., Postan, Miriam, Martin, Diana, Fralish, Bolyn Hubby, Albareda, Maria C., Alvarez, Maria G., Lococo, Bruno, Barbieri, Gustavo, Viotti, Rodolfo J., Tarleton, Rick L.
Format:	Journal Article
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-03-2004 University of Chicago Press Oxford University Press
Subjects:	Animals Biological and medical sciences Biomarkers - blood Cardiomyopathies CD8-Positive T-Lymphocytes - immunology Chagas disease Chagas Disease - immunology Chagas Disease - physiopathology Chronic diseases Epitopes Fundamental and applied biological sciences. Psychology Histocompatibility Testing HLA-A2 Antigen - chemistry HLA-A2 Antigen - immunology Human protozoal diseases Humans Infections Infectious diseases Interferon-gamma - immunology Medical sciences Microbiology Parasites Parasitic diseases Parasitism Peptide Fragments - chemistry Peptide Fragments - immunology Protozoal diseases Reference Values Severity of Illness Index T lymphocytes T-Lymphocytes - immunology T-Lymphocytes, Cytotoxic - immunology Transponders Trypanosoma cruzi Trypanosoma cruzi - immunology Trypanosomiasis Kinetoplastida Human Protozoa Protozoal disease Microbiology Cytokine Chagas disease Parasitosis Trypanosoma cruzi Infection Chronic T-Lymphocyte Trypanosomiasis Gamma interferon
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Summary:	This study sought to quantify CD8+ T cell responses to Trypanosoma cruzi and to identify potential links between these responses and the severity of disease in humans. In the majority of patients with Chagas disease, staining with class I major histocompatibility complex tetramers and analysis of interferon (IFN)-γ ELISPOT responses to a panel of known cytotoxic T lymphocyte target epitopes from T. cruzi failed to identify parasitespecific CD8+ T cells. However, the frequency of individuals with positive ELISPOT responses was higher in areas of active transmission. Analysis of IFN-γ ELISPOT responses to a parasite lysate revealed a very high frequency of responders among patients with mild clinical disease and a very low frequency of responders among those with the most severe form of the disease. These data suggest that the frequency of IFN-γ- producing T cells in patients with chronic Chagas disease is associated with the history of recent exposure and with the clinical status of the patient.
Bibliography:	Present affiliation: AlphaVax, Inc., Research Triangle Park, North Carolina. istex:75BF2878C94F123B9856128BFFF16BF5333C57AE ark:/67375/HXZ-425BSR39-4 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1899 1537-6613
DOI:	10.1086/381682