Modulation of cell death mechanisms via α-Ag2WO4 morphology-dependent factors
The cytotoxic of α-Ag2WO4 synthesized in different morphologies (cuboidal (AW-C), hexagonal rod-like (AW-HRL) and nanometric rod-like (AW-NRL) was analyzed to understand the impact of morphological modulation on the toxicity of 3 T3 cell lines in the dark and when photoactivated by visible light. Pa...
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| Published in: | Journal of photochemistry and photobiology. B, Biology Vol. 257; p. 112947 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier B.V
01-08-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The cytotoxic of α-Ag2WO4 synthesized in different morphologies (cuboidal (AW-C), hexagonal rod-like (AW-HRL) and nanometric rod-like (AW-NRL) was analyzed to understand the impact of morphological modulation on the toxicity of 3 T3 cell lines in the dark and when photoactivated by visible light. Pathways of toxicity were examined, such as parameters and electrostatic interaction, uptake, ion release and ROS production. Cytotoxicity was observed for all samples after reaching concentrations exceeding 7.8 μg/mL. Uptake tests demonstrated that the samples were not internalized by cells, likely due to their negative surface charge. AW-NRL exhibited autophagy in the absence of light and during photoactivation, primarily attributed to its ability to generate singlet oxygen. Analyzing intercellular ROS and RNS production, AW-HRL induced an increase in NO through exposure to photo-generated hydroxyl radicals, while AW-NRL showed increases only at non-photoactivated concentrations and AW-C did not exhibit increases. Interestingly, in the dark, these cells showed a low propensity for apoptosis, with late apoptosis and necrosis being more pronounced. When photoactivated, this behavior changed, revealing predominantly apoptotic and late apoptotic cell death. There is a need for an understanding of how morphology can alter the biological properties of α-Ag2WO4 to predict and optimize its effects on cellular responses.
The morphological modulation of α-Ag2WO4 induces physicochemical modifications in this material, leading to distinct cytotoxic effects for each morphology.
This proves to be an effective strategy for modulating the biological response of materials of this kind. [Display omitted]
•Three orthorhombic particles of α-Ag2WO4 were synthesized through three distinct methods and each of them exhibits distinct effects on fibroblasts.•The effects on cells are significant, positioning α-Ag2WO4 as a potential agent against eukaryotic cells.•Each morphology induces cell death through apoptosis or necrosis in distinct ways. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1011-1344 1873-2682 1873-2682 |
| DOI: | 10.1016/j.jphotobiol.2024.112947 |