Protection against Toxoplasmosis in Mice Immunized with Different Antigens of Toxoplasma gondii Incorporated into Liposomes

Different toxoplasma antigens were entrapped within liposomes and evaluated, in this form, for their ability to protect Swiss mice against toxoplasma infection: soluble tachyzoite antigen (L/TAg), tissue cyst (L/CAg), tachyzoite plus tissue cyst (L/TCAg) or purified antigen of tachyzoite (L/pTAg). T...

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Bibliographic Details
Published in:	Memórias do Instituto Oswaldo Cruz Vol. 94; no. 4; pp. 485 - 490
Main Authors:	Elsaid, M M, Vitor, R W, Frézard, F J, Martins, M S
Format:	Journal Article
Language:	English
Published:	Brazil Fundação Oswaldo Cruz, Fiocruz 01-07-1999 Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	Animals Antibodies, Monoclonal Antigens, Protozoan - administration & dosage Antigens, Protozoan - isolation & purification Brazil Dose-Response Relationship, Immunologic Drug Carriers Female immunoprotection Liposomes Mice PARASITOLOGY Protozoan Proteins - immunology Protozoan Vaccines - immunology purified antigen toxoplasma Toxoplasma - immunology toxoplasma - immunoprotection - purified antigen - mice - Brazil Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis - immunology Toxoplasmosis - prevention & control TROPICAL MEDICINE Brazil purified antigen immunoprotection mice toxoplasma
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Summary:	Different toxoplasma antigens were entrapped within liposomes and evaluated, in this form, for their ability to protect Swiss mice against toxoplasma infection: soluble tachyzoite antigen (L/TAg), tissue cyst (L/CAg), tachyzoite plus tissue cyst (L/TCAg) or purified antigen of tachyzoite (L/pTAg). The protein used in L/pTAg was purified from tachyzoites using a stage-specific monoclonal antibody which reacted at a molecular weight of 32 kD in SDS PAGE and silver stain using reduced condition. To compare the immuno-adjuvant action of liposomes and of Freund's Complete Adjuvant (FCA), another group of mice was immunized with soluble tachyzoite antigen (STAg) emulsified in FCA (FCA/TAg). Control groups were inoculated with (STAg) alone, phosphate-buffered saline (PBS), FCA with PBS (FCA/PBS) and empty liposomes (L/PBS). Mice were inoculated subcutaneously with these antigens six, four and two weeks before a challenge with 80 tissue cysts of the P strain of Toxoplasma gondii orally. All mice immunized with or without adjuvant showed a humoral response, as measured by Elisa. However, no correlation was found between antibody titer and protection against the challenge. All mice immunized with L/pTAg or L/TCAg survived (100), whereas 80% and 90% of mice from groups which received respectively PBS or FCA/PBS and L/PBS died. All mice immunized with antigens entrapped within liposomes (L/TAg, L/CAg, L/TCAg and L/pTAg) showed low numbers of intracerebral cysts.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1678-8060 0074-0276 0074-0276 1678-8060
DOI:	10.1590/S0074-02761999000400010