The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination

The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination

The influence of abscisic acid (ABA) on the processes of formation of different polysomal populations, their structures and stability in embryonal tissue during pea seeds germination was studied. The contents of total ribosomal fraction increased in all samples up to 72 h of germination and then dec...

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Published in:Acta physiologiae plantarum Vol. 25; no. 1; pp. 5 - 12
Main Authors: Weidner, S, Fr¹czek, E, Romanowska, M. (University of Warmia and Mazury, Olsztyn (Poland). Dept. of Biochemistry), Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science), Abe, S. (Ehime University (Japan). Lab. of Moleculary Cell Biology), Davies, E. (North Carolina State University, Raleigh (USA). Dept. of Botany)
Format: Journal Article
Language:English
Published: 01-01-2003
Subjects:
ABA
AISLAMIENTO
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
EMBRYO CULTURE
FRACCIONAMIENTO
FRACTIONATION
FRACTIONNEMENT
GERMINACIÓN
GERMINATION
GRAINE
GUISANTE
ISOLATION
ISOLEMENT
PEAS
PISUM SATIVUM
POIS
RIBOSOMAS
RIBOSOME
RIBOSOMES
SEEDS
SEMILLA
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Summary:The influence of abscisic acid (ABA) on the processes of formation of different polysomal populations, their structures and stability in embryonal tissue during pea seeds germination was studied. The contents of total ribosomal fraction increased in all samples up to 72 h of germination and then decreased. The contents of polysomal population (FP, MBP, CBP and CMBP) extracted from the embryonal tissue after 72 hrs of germination of pea seeds were then quantified. It turned out that in examined tissue of control sample, fraction of free polysomes (FP) was the most abounded. This population of polysomes in sprouts decreased after ABA treatment. FP content decreased even more when the higher ABA concentration was applied during germination. Similar changes were observed in the fraction of membrane-bound polysomes (MBP). Quite different tendencies were found, however, in forming population of the cytoskeleton-membrane-bound polysomes (CMBP). The CMBP population content in embryonal tissue increased in a dosage dependent manner with increasing concentration of ABA applied during seed germination. This indicates the important role of CMBP fraction in synthesis of specific proteins in embryos in the time when processes of seeds germination are retarded by ABA. In the final part we examined the stability of polysomes isolated from sprouts of germinating seeds in water and sprouts isolated from seeds treated with ABA (100 uM) during germination. Total polysomes isolated from embryonal tissue of germinating seeds treated with ABA showed much higher resistance to exogenous ribonuclease digestion than total polysomes of control sample. The obtained results suggest that ABA influence on different polysomal population formation also controls their stability
Bibliography:2003001979
F30
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-003-0030-3