Tropism and virulence of Cutibacterium (formerly Propionibacterium) acnes involved in implant-associated infection

The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes cli...

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Published in:	Anaerobe Vol. 47; pp. 73 - 78
Main Authors:	Aubin, Guillaume Ghislain, Lavigne, Jean-Philippe, Foucher, Yohan, Dellière, Sarah, Lepelletier, Didier, Gouin, François, Corvec, Stéphane
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-10-2017 Elsevier Masson
Subjects:	Animals Caenorhabditis elegans Caenorhabditis elegans - microbiology Caenorhabditis elegans - physiology Cancer Cutibacterium (Propionibacterium) acnes Disease Models, Animal Diversilab Gram-Positive Bacterial Infections - microbiology Humans Life Sciences Multi-Locus Sequence Typing Multilocus Sequence Typing Propionibacterium acnes - classification Propionibacterium acnes - genetics Propionibacterium acnes - pathogenicity Propionibacterium acnes - physiology Prosthesis-Related Infections - microbiology Prosthetic joint infections Survival Analysis Viral Tropism Virulence Virulence Factors - analysis Virulence Factors - genetics Whole Genome Sequencing Multi-Locus Sequence Typing Caenorhabditis elegans Whole genome sequencing Virulence Diversilab Prosthetic joint infections Cutibacterium (Propionibacterium) acnes Diversilab(®)
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Summary:	The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates through Multi-Locus Sequence Typing (MLST), we established a Caenorhabditis elegans model to assess C. acnes virulence and we investigated the presence of virulence factors in our collection. Ours results showed that CC36 and CC53 C. acnes isolates were more frequently observed in prosthetic joint infections (PJI) than CC18 and CC28 C. acnes isolates (p = 0.021). The C. elegans model developed here showed two distinct virulence groups of C. acnes (p < 0.05). These groups were not correlated to CC or clinical origin. Whole genome sequencing allowed us to identify a putative gene linked to low virulent strains. In conclusion, MLST remains a good method to screen pathogenic C. acnes isolates according to their clinical context but mechanisms of C. acnes virulence need to be assess thought transcriptomic analysis to investigate regulatory process. •C. acnes isolates belonging to clonal complexes (CC) 36 and CC53 are linked to prosthetic joint infections.•C. acnes isolates exhibit two distinct virulence behaviours not linked to CC or clinical origin.•C. elegans a useful model to assess the virulence of C. acnes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1075-9964 1095-8274
DOI:	10.1016/j.anaerobe.2017.04.009