Umbelliferone reduces inflammation and ligature‐induced osteoclastic alveolar bone resorption in mice

Umbelliferone reduces inflammation and ligature‐induced osteoclastic alveolar bone resorption in mice

Aims This study aimed to investigate the effects of Umbelliferone (UMB) on the inflammation underlying alveolar bone resorption in mouse periodontitis. Methods Male Swiss mice subjected to a ligature of molars were grouped as non‐treated (NT), received UMB (15, 45, or 135 mg/kg) or saline daily for...

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Published in:Journal of periodontal research Vol. 59; no. 5; pp. 982 - 992
Main Authors: Tavares, Samia Jessica Silva, Pereira, Camila Rodrigues, Fortes, Roseane Aline Monteiro, Alves, Bianca Elen Souza, Fonteles, Cristiane Sá Roriz, Wong, Deysi Viviana Tenazoa, Lima‐Júnior, Roberto César Pereira, Moraes, Manoel Odorico, Lima, Vilma
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-10-2024
Subjects:
Acid phosphatase
Acid phosphatase (tartrate-resistant)
Alveolar bone
Body mass
Bone histomorphometry
Bone mass
Bone resorption
Cathepsin K
coumarins
cytokine
Cytokines
Dental enamel
Enzyme-linked immunosorbent assay
Gum disease
Immunofluorescence
Immunohistochemistry
Inflammation
Molars
NF‐kappa B
Osteoclasts
Periodontitis
Peroxidase
Root resorption
tartrate‐resistant acid phosphatase
Umbelliferone
cytokine
NF‐kappa B
cathepsin K
coumarins
tartrate‐resistant acid phosphatase
periodontitis
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Summary:Aims This study aimed to investigate the effects of Umbelliferone (UMB) on the inflammation underlying alveolar bone resorption in mouse periodontitis. Methods Male Swiss mice subjected to a ligature of molars were grouped as non‐treated (NT), received UMB (15, 45, or 135 mg/kg) or saline daily for 7 days, respectively, and were compared with naïve mice as control. Gingival tissues were evaluated by myeloperoxidase (MPO) activity and interleukin‐1β level by ELISA. The bone resorption was directly assessed on the region between the cement–enamel junction and the alveolar bone crest. Microscopically, histomorphometry of the furcation region, immunofluorescence for nuclear factor‐kappa B (NF‐ĸB), and immunohistochemistry for tartrate‐resistant acid phosphatase (TRAP), and cathepsin K (CTSK) were performed. Systemically, body mass variation and leukogram were analyzed. Results Periodontitis significantly increased MPO activity, interleukin‐1β level, and NF‐ĸB+ immunofluorescence, and induced severe alveolar bone and furcation resorptions, besides increased TRAP+ and CTSK+ cells compared with naïve. UMB significantly prevented the inflammation by reducing MPO activity, interleukin‐1β level, and NF‐ĸB+ intensity, besides reduction of resorption of alveolar bone and furcation area, and TRAP+ and CTSK+ cells compared with the NT group. Periodontitis or UMB treatment did not affect the animals systemically. Conclusion UMB improved periodontitis by reducing inflammation and bone markers. The effects of umbelliferone in mice with ligature‐induced periodontitis. Periodontitis increased MPO activity, IL‐1β level, NF‐ĸB, TRAP, and CTSK cells compared to healthy periodontium. UMB prevented inflammation by reducing MPO activity, IL‐1β, and NF‐ĸB levels and decreasing alveolar bone resorption, with reduced TRAP and CTSK expressions.
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ISSN:0022-3484
1600-0765
1600-0765
DOI:10.1111/jre.13277