Automated high-throughput high-content autophagy and mitophagy analysis platform

Automated high-throughput high-content autophagy and mitophagy analysis platform

Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new to...

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Bibliographic Details
Published in:Scientific reports Vol. 9; no. 1; pp. 9455 - 11
Main Authors: Arias-Fuenzalida, Jonathan, Jarazo, Javier, Walter, Jonas, Gomez-Giro, Gemma, Forster, Julia I., Krueger, Rejko, Antony, Paul M. A., Schwamborn, Jens C.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01-07-2019
Nature Publishing Group
Subjects:
13/100
13/106
13/56
14/1
14/19
14/35
631/532/2064/2158
631/80/39/2348
Algorithms
Automation
Autophagosomes - metabolism
Autophagy
Autophagy - drug effects
Chloroquine - pharmacology
Homeostasis
Humanities and Social Sciences
Humans
Image processing
Image Processing, Computer-Assisted
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Intermediates
Lysosomes - metabolism
Microscopy, Fluorescence
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Mitophagy
Mitophagy - drug effects
multidisciplinary
Phagocytosis
Phenotyping
Science
Science (multidisciplinary)
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Description
Summary:Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technologically challenging and has not been addressed yet. Here, we overcome those requirements and limitations by the developed of stable autophagy and mitophagy reporter-iPSC and the establishment of a novel high-throughput phenotyping platform utilizing automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-45917-2