Tissue-specific transcriptional regulation of epithelial/endothelial and mesenchymal markers during renovascular hypertension

Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition are processes that can occur under different biological conditions, including tissue healing due to hypertension and oxidative stress. The purpose of the present study was to evaluate the differences in gene express...

Full description

Saved in:

Bibliographic Details
Published in:	Molecular medicine reports Vol. 20; no. 5; pp. 4467 - 4476
Main Authors:	Ferraz, Leandro Ricardo, Moreira, Bianca Caruso, de Queiroz, Gabriela de Souza Rodrigues, Formigari, Regiane de Freitas, Esquisatto, Marcelo Augusto Marreto, Felonato, Maira, Alves, Armindo Antonio, Thomazini, Bruna Fontana, de Oliveira, Camila Andréa
Format:	Journal Article
Language:	English
Published:	Greece Spandidos Publications 01-11-2019 Spandidos Publications UK Ltd D.A. Spandidos
Subjects:	Actin Analysis Animals Antigens, Differentiation - biosynthesis Atrophy Biochemistry Blood pressure Bone morphogenetic proteins Cell adhesion & migration Collagen Collagen (type I) Collagen (type III) E-cadherin Endothelium Experiments Gene expression Gene regulation Genes Genetic aspects Heart Heart hypertrophy Hepatocyte growth factor Hypertension Hypertension, Renal - metabolism Hypertension, Renal - pathology Hypertrophy Inflammation Interleukin 10 Interleukin 6 Interleukins Kidneys Laboratory animals Liver Male Mesenchyme Muscle proteins N-Cadherin Necrosis Organ Specificity Oxidative stress Proteins Rats Rats, Wistar Renal artery Renovascular hypertension Reverse transcription Scientific equipment industry Smooth muscle Stem cells Thiobarbituric acid Thiols Transcription (Genetics) Transcriptional Activation Tumor necrosis factor-TNF Tumor necrosis factor-α Tumors Veins & arteries Brazil United States
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition are processes that can occur under different biological conditions, including tissue healing due to hypertension and oxidative stress. The purpose of the present study was to evaluate the differences in gene expression of epithelial/endothelial and mesenchymal markers in different tissues. A two-kidney, one-clip (2K1C) renovascular hypertension rat model was used. Hypertension was induced by the clipping of the left renal artery; the rats were randomized into sham and 2K1C groups and monitored for up to 4 weeks. The gene expressions of E-cadherin (E-cad), N-cadherin (N-cad), α-smooth muscle actin (α-SMA), collagen I (COL1A1), collagen III (COL3A1) and hepatocyte growth factor (HGF) were determined by reverse transcription-PCR. The levels of the cytokines transforming growth factor-β1, tumor necrosis factor-α, interleukin (IL)-4, IL-6 and IL-10 were evaluated using ELISAs. The levels of thiobarbituric acid reactive substances and thiol groups were measured to evaluate oxidative stress. All analyses were performed on the liver, heart and kidneys tissues of sham and model rats. The 2K1C animals exhibited a higher systolic blood pressure, as well as cardiac hypertrophy and atrophy of the left kidney. Fibrotic alterations in the heart and kidneys were observed, as was an increase in the collagen fiber areas, and higher levels of inflammatory cytokines, which are associated with the increased expression of fibroproliferative and anti-fibrotic genes. Renovascular hypertension regulated epithelial/endothelial and mesenchymal markers, including E-cad, N-cad, α-SMA and COL1A1 in the kidneys and heart. EMT in the kidneys was mediated by an increased level of inflammatory and profibrotic cytokines, as well as by oxidative stress. The data in the present study suggested that the expression of epithelial/endothelial and mesenchymal markers are differentially regulated by hypertension in the liver, heart and kidneys.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1791-2997 1791-3004
DOI:	10.3892/mmr.2019.10722