Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae

A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties....

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Bibliographic Details
Published in:	FEBS letters Vol. 582; no. 29; pp. 4032 - 4038
Main Authors:	Maury, Jérôme, Asadollahi, Mohammad A., Møller, Kasper, Schalk, Michel, Clark, Anthony, Formenti, Luca R., Nielsen, Jens
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 10-12-2008
Subjects:	2-C-methyl-d-erythritol 4-phosphate 2-Methyl erythritol 4-phosphate Cloning, Molecular dimethyl allyl diphosphate DMAPP Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism G3P gas chromatography–mass spectrometry GC–MS glyceraldehyde 3-phosphate IPP isopentenyl diphosphate Isoprenoid Lovastatin - pharmacology MEP Mevalonate MVA Plasmids reverse transcriptase polymerase chain reaction RT-PCR Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Terpenes - metabolism Yeast GC–MS Yeast Mevalonate G3P RT-PCR IPP MEP MVA DMAPP Isoprenoid Saccharomyces cerevisiae 2-Methyl erythritol 4-phosphate
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Summary:	A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468 1873-3468
DOI:	10.1016/j.febslet.2008.10.045