Search Results - "Forkey, Joseph N."
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Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization
Published in Nature (London) (27-03-2003)“…The structural change that generates force and motion in actomyosin motility has been proposed to be tilting of the myosin light chain domain, which serves as…”
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2
Orientation of the Myosin Light Chain Region by Single Molecule Total Internal Reflection Fluorescence Polarization Microscopy
Published in Biophysical journal (01-08-2005)“…To study the orientation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensembles of myosin decorating actin filaments…”
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3
Myosin V Walks Hand-over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Published in Science (American Association for the Advancement of Science) (27-06-2003)“…Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ~37 nanometers for each adenosine triphosphate…”
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4
Rotational Motions of Macro- molecules by Single-Molecule Fluorescence Microscopy
Published in Accounts of chemical research (01-07-2005)“…Several complementary techniques have been developed to determine average orientation, dynamics on multiple time scales, and concerted rotational motions of…”
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Measurement of Single Macromolecule Orientation by Total Internal Reflection Fluorescence Polarization Microscopy
Published in Biophysical journal (01-08-2005)“…A new approach is presented for measuring the three-dimensional orientation of individual macromolecules using single molecule fluorescence polarization (SMFP)…”
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Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM)
Published in Cold Spring Harbor protocols (01-05-2012)“…In this article, we describe methods to detect the spatial orientation and rotational dynamics of single molecules using polarized total internal reflection…”
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The acquisition and analysis of polarized total internal reflection fluorescence microscopy (polTIRFM) data
Published in Cold Spring Harbor protocols (01-06-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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Fluorescent labeling of calmodulin with bifunctional rhodamine
Published in Cold Spring Harbor protocols (01-05-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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Preparation of filamentous actin for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays
Published in Cold Spring Harbor protocols (01-05-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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The polarized total internal reflection fluorescence microscopy (polTIRFM) processive motility assay for myosin V
Published in Cold Spring Harbor protocols (01-06-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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11
Construction of flow chambers for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays
Published in Cold Spring Harbor protocols (01-06-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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12
Fluorescent labeling of myosin V for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays
Published in Cold Spring Harbor protocols (01-05-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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13
The polarized total internal reflection fluorescence microscopy (polTIRFM) twirling filament assay
Published in Cold Spring Harbor protocols (01-06-2012)“…Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single…”
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14
Protein structural dynamics by single-molecule fluorescence polarization
Published in Progress in Biophysics and Molecular Biology (01-01-2000)Get full text
Book Review Journal Article -
15
Measurement of Single Macromolecule Orientation by Total Internal Reflection Fluorescence Polarization Microscopy
Published in Biophysical journal (01-08-2005)Get full text
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Kinesin-ADP: whole lotta shakin' goin' on
Published in Nature structural biology (01-06-2001)Get full text
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Single molecule polarization microscopy for investigating real time structural dynamics of biological macromolecules
Published in Proceedings of the First Joint BMES/EMBS Conference : serving humanity advancing technology, Oct. 13-16, 99, Atlanta, GA, USA (1999)“…To quantitatively monitor structural dynamics of individual biomolecules in real time, a single-molecule polarization microscopy technique has been developed…”
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Conference Proceeding Journal Article