1000 spider silkomes: Linking sequences to silk physical properties

Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obta...

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Bibliographic Details
Published in:	Science advances Vol. 8; no. 41; p. eabo6043
Main Authors:	Arakawa, Kazuharu, Kono, Nobuaki, Malay, Ali D., Tateishi, Ayaka, Ifuku, Nao, Masunaga, Hiroyasu, Sato, Ryota, Tsuchiya, Kousuke, Ohtoshi, Rintaro, Pedrazzoli, Daniel, Shinohara, Asaka, Ito, Yusuke, Nakamura, Hiroyuki, Tanikawa, Akio, Suzuki, Yuya, Ichikawa, Takeaki, Fujita, Shohei, Fujiwara, Masayuki, Tomita, Masaru, Blamires, Sean J., Chuah, Jo-Ann, Craig, Hamish, Foong, Choon P., Greco, Gabriele, Guan, Juan, Holland, Chris, Kaplan, David L., Sudesh, Kumar, Mandal, Biman B., Norma-Rashid, Y., Oktaviani, Nur A., Preda, Rucsanda C., Pugno, Nicola M., Rajkhowa, Rangam, Wang, Xiaoqin, Yazawa, Kenjiro, Zheng, Zhaozhu, Numata, Keiji
Format:	Journal Article
Language:	English
Published:	American Association for the Advancement of Science 14-10-2022
Subjects:	Biomedicine and Life Sciences Genetics Materials Science SciAdv r-articles
Online Access:	Get full text
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Summary:	Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs. The combination of spider silk genotype-phenotype data revealed essential amino acid motifs contributing to physical properties.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: NMR Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW 2052, Australia. These authors contributed equally to this work.
ISSN:	2375-2548 2375-2548
DOI:	10.1126/sciadv.abo6043