Inhibition of breast cancer xenografts in a mouse model and the induction of apoptosis in multiple breast cancer cell lines by lactoferricin B peptide

Inhibition of breast cancer xenografts in a mouse model and the induction of apoptosis in multiple breast cancer cell lines by lactoferricin B peptide

Breast cancer has a diverse aetiology characterized by the heterogeneous expression of hormone receptors and signalling molecules, resulting in varied sensitivity to chemotherapy. The adverse side effects of chemotherapy coupled with the development of drug resistance have prompted the exploration o...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine Vol. 25; no. 15; pp. 7181 - 7189
Main Authors: Rahman, Rizdwan, Fonseka, Alyssa Danielle, Sua, Shiang‐Chia, Ahmad, Munirah, Rajendran, Ramkumar, Ambu, Stephen, Davamani, Fabian, Khoo, Alan Soo‐Beng, Chitra, Ebenezer
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01-08-2021
John Wiley and Sons Inc
Subjects:
Animals
anticancer peptide
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Apoptosis
Apoptosis - drug effects
Breast cancer
Cancer therapies
Cell cycle
Cell Line
Chemotherapy
Committees
Drug dosages
Drug resistance
Epidermal growth factor
Epithelial cells
Female
Growth rate
Humans
Kinases
lactoferricin B
Lactoferrin
Lactoferrin - pharmacology
Lactoferrin - therapeutic use
Lymphoma
Mammary Neoplasms, Experimental - drug therapy
MCF-7 Cells
Medical prognosis
Medical research
Mice
Mice, Inbred NOD
Mice, SCID
milk peptide
natural peptide
Natural products
Original
Penicillin
Peptides
Tumor cell lines
Tumors
Variance analysis
Xenografts
breast cancer
anticancer peptide
milk peptide
natural peptide
lactoferricin B
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Summary:Breast cancer has a diverse aetiology characterized by the heterogeneous expression of hormone receptors and signalling molecules, resulting in varied sensitivity to chemotherapy. The adverse side effects of chemotherapy coupled with the development of drug resistance have prompted the exploration of natural products to combat cancer. Lactoferricin B (LfcinB) is a natural peptide derived from bovine lactoferrin that exhibits anticancer properties. LfcinB was evaluated in vitro for its inhibitory effects on cell lines representing different categories of breast cancer and in vivo for its suppressive effects on tumour xenografts in NOD‐SCID mice. The different breast cancer cell lines exhibited varied levels of sensitivity to apoptosis induced by LfcinB in the order of SKBR3>MDA‐MB‐231>MDA‐MB‐468>MCF7, while the normal breast epithelial cells MCF‐10A were not sensitive to LfcinB. The peptide also inhibited the invasion of the MDA‐MB‐231 and MDA‐MB‐468 cell lines. In the mouse xenograft model, intratumoural injections of LfcinB significantly reduced tumour growth rate and tumour size, as depicted by live imaging of the mice using in vivo imaging systems (IVIS). Harvested tumour volume and weight were significantly reduced by LfcinB treatment. LfcinB, therefore, is a promising and safe candidate that can be considered for the treatment of breast cancer.
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ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.16748