The role of oxidative stress in alterations of hematological parameters and inflammatory markers induced by early hypercholesterolemia

The investigation of the effects of a high cholesterol diet (HD) for a short-time period on hematological parameters and the potential role of oxidative stress and inflammation markers. Rabbits were fed either a control diet or a diet containing 1% cholesterol (HD) for 5–6weeks. The plasma lipid lev...

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Published in:	Life sciences (1973) Vol. 93; no. 15; pp. 503 - 508
Main Authors:	Karbiner, María Sofía, Sierra, Liliana, Minahk, Carlos, Fonio, María Cristina, Bruno, María Peral de, Jerez, Susana
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 10-10-2013
Subjects:	Anemia Animals Antioxidants - metabolism Biomarkers - blood Blood Cell Count Blood cells blood lipids blood platelet count blood serum C reactive protein cardiovascular diseases cholesterol Cholesterol, Dietary - adverse effects diet erythrocytes glutathione hematocrit Hypercholesterolemia Hypercholesterolemia - blood Hypercholesterolemia - physiopathology inflammation Inflammation Mediators - blood Male Membrane fluidity Membrane Fluidity - physiology neutrophils Osmotic Fragility - physiology Oxidative stress Oxidative Stress - physiology Rabbit Rabbits thiobarbituric acid-reactive substances Oxidative stress Membrane fluidity Hypercholesterolemia Anemia Blood cells Rabbit C reactive protein
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Summary:	The investigation of the effects of a high cholesterol diet (HD) for a short-time period on hematological parameters and the potential role of oxidative stress and inflammation markers. Rabbits were fed either a control diet or a diet containing 1% cholesterol (HD) for 5–6weeks. The plasma lipid levels, C reactive protein (CRP), total red blood cells (RBC), total white blood cells (WBC), platelet count, packed cell volume (PCV) and leukocyte formula were determined. Oxidative stress was evaluated by the thiobarbituric acid reactive substances (TBARS), total glutathione and GSH serum level measurements. The osmotic fragility and the membrane fluidity of erythrocytes were determined. The levels of total cholesterol and TBARS were also measured in the erythrocyte membrane suspension. A decrease in the RBC and PCV was observed in rabbits fed on HD. The membrane rigidity and osmotic fragility were increased, and the morphological changes caused by the HD and TBARS levels in the erythrocyte membrane may account for this phenomenon. The inflammatory markers as the CRP levels, the platelet count, the WBC and the neutrophils were increased. The TBARS and GSH levels in the serum were increased and decreased, respectively. This study shows that feeding rabbits an HD for a short time induces hematological alterations, disturbances in the oxidant–antioxidant balance and an increase of inflammatory markers. These findings support the importance of the early correction or prevention of high cholesterol levels to disrupt the process leading to the development of cardiovascular diseases.
Bibliography:	http://dx.doi.org/10.1016/j.lfs.2013.08.003 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0024-3205 1879-0631
DOI:	10.1016/j.lfs.2013.08.003