Clonal Characterization of a Bipotent T Cell and NK Cell Progenitor in the Mouse Fetal Thymus

We recently described a population of fetal thymocytes with a CD117+NK1.1+CD90lowCD25- phenotype, which were shown to contain committed T cell and NK cell progenitors. However, the characterization of a single cell with a restricted T and NK cell precursor potential was lacking. Here, using an in vi...

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Bibliographic Details
Published in:	The Journal of immunology (1950) Vol. 164; no. 4; pp. 1730 - 1733
Main Authors:	Michie, Alison M, Carlyle, James R, Schmitt, Thomas M, Ljutic, Belma, Cho, Sarah K, Fong, Quyen, Zuniga-Pflucker, Juan Carlos
Format:	Journal Article
Language:	English
Published:	United States Am Assoc Immnol 15-02-2000
Subjects:	Animals Antigens - biosynthesis Antigens, Ly Antigens, Surface CD117 antigen CD25 antigen CD90 antigen Cell Lineage - immunology Clone Cells Colony-Forming Units Assay Immunophenotyping Killer Cells, Natural - cytology Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Lectins, C-Type Mice Mice, Inbred C57BL NK Cell Lectin-Like Receptor Subfamily B NK1.1 antigen Organ Culture Techniques Protein Biosynthesis Proteins Proto-Oncogene Proteins c-kit - biosynthesis Stem Cells - cytology Stem Cells - immunology Stem Cells - metabolism T-Lymphocyte Subsets - cytology T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism Thymus Gland - cytology Thymus Gland - embryology Thymus Gland - immunology
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Summary:	We recently described a population of fetal thymocytes with a CD117+NK1.1+CD90lowCD25- phenotype, which were shown to contain committed T cell and NK cell progenitors. However, the characterization of a single cell with a restricted T and NK cell precursor potential was lacking. Here, using an in vitro model for T and NK cell differentiation, we provide conclusive evidence demonstrating the existence of a clonal lineage-restricted T and NK cell progenitor. These results establish that fetal thymocytes with a CD117+NK1.1+CD90lowCD25- phenotype represent bipotent T and NK cell progenitors.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.164.4.1730