Selenoprotein I is essential for murine embryogenesis

Selenoprotein I is essential for murine embryogenesis

Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI−/+ mating pairs were used to generate global KO...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics Vol. 689; p. 108444
Main Authors: Avery, Joseph C., Yamazaki, Yukiko, Hoffmann, FuKun W., Folgelgren, Benjamin, Hoffmann, Peter R.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 15-08-2020
Subjects:
Animals
Animals, Newborn
Blastocyst - pathology
Blastocyst - ultrastructure
Embryo Implantation
Embryo Loss - genetics
Embryo Loss - pathology
Embryonic Development
Embryonic lethal
Ethanolaminephosphotransferase
Female
Gene Deletion
Gene Expression Regulation, Developmental
Homozygote
Male
Mice - embryology
Mice - genetics
Mice, Inbred C57BL
Mice, Knockout
Phosphatidylethanolamine
Phospholipid transferase
Pregnancy
Selenium
ethanolamine kinase 1
knockout
Phosphatidylethanolamine
glycophosphatidylinositol
ETP-phosphoethanolamine cytidyltransferase
selenoprotein I
Phospholipid transferase
phosphatidylserine
parts per million
Embryonic lethal
haematoxylin and eosin
floxed
Selenium
wild-type
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Summary:Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI−/+ mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ~ E6, blastocyst embryos (E3.5-E4.4) were flushed from uteruses of pregnant females and analyzed for morphology and genotype. KO embryos were detected in 5 of 6 pregnant females, and 7 of the 32 genotyped embryos were found to be SELENOI KO that exhibited no overt pathological features. Overall, these results demonstrate that, except for rare cases (2/490 = 0.4%), global SELENOI deletion leads to early embryonic lethality.
ISSN:0003-9861
1096-0384
DOI:10.1016/j.abb.2020.108444