Determination of toxinotypes of environmental Clostridium perfringens by Polymerase Chain Reaction

Toxinotype of Clostridium perfringens (CP) isolates collected from the Bernam River, Selangor River and Tengi Canal between April 2007 and January 2008 were determined by Polymerase Chain Reaction (PCR) using published primers. All the 147 isolates were toxinotype Type A, harbouring the alpha toxin...

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Bibliographic Details
Published in:	Tropical biomedicine Vol. 28; no. 1; pp. 171 - 174
Main Authors:	Florence L, C H, Hakim, S L, Kamaluddin, M A, Thong, K L
Format:	Journal Article
Language:	English
Published:	Malaysia 01-04-2011
Subjects:	Bacterial Toxins - classification Bacterial Toxins - genetics Bacteriological Techniques - methods Clostridium perfringens - classification Clostridium perfringens - genetics Clostridium perfringens - pathogenicity DNA Primers - genetics Genotype Humans Malaysia Polymerase Chain Reaction - methods Rivers - microbiology Malaysia
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Summary:	Toxinotype of Clostridium perfringens (CP) isolates collected from the Bernam River, Selangor River and Tengi Canal between April 2007 and January 2008 were determined by Polymerase Chain Reaction (PCR) using published primers. All the 147 isolates were toxinotype Type A, harbouring the alpha toxin gene. In addition, 5 of the isolates also had the enterotoxin (CPE) gene.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0127-5720