Search Results - "Floor, Robert J."

Structure- and sequence-analysis inspired engineering of proteins for enhanced thermostability by Wijma, Hein J, Floor, Robert J, Janssen, Dick B

“…•Targeting critical sites for stability improvement.•Consensus/ancestral sequence based protein stabilization.•Computational design of thermostability.•Loop…”
Get full text

Computational Library Design for Increasing Haloalkane Dehalogenase Stability by Floor, Robert J., Wijma, Hein J., Colpa, Dana I., Ramos-Silva, Aline, Jekel, Peter A., Szymański, Wiktor, Feringa, Ben L., Marrink, Siewert J., Janssen, Dick B.

“…We explored the use of a computational design framework for the stabilization of the haloalkane dehalogenase LinB. Energy calculations, disulfide bond design,…”
Get full text

Directed Evolution Strategies for Enantiocomplementary Haloalkane Dehalogenases: From Chemical Waste to Enantiopure Building Blocks by van Leeuwen, Jan G. E., Wijma, Hein J., Floor, Robert J., van der Laan, Jan-Metske, Janssen, Dick B.

“…We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that convert the toxic waste compound 1,2,3‐trichloropropane (TCP)…”
Get full text

Enantioselective Enzymes by Computational Design and In Silico Screening by Wijma, Hein J., Floor, Robert J., Bjelic, Sinisa, Marrink, Siewert J., Baker, David, Janssen, Dick B.

“…Computational enzyme design holds great promise for providing new biocatalysts for synthetic chemistry. A strategy to design small mutant libraries of…”
Get full text

Computationally designed libraries for rapid enzyme stabilization by Wijma, Hein J., Floor, Robert J., Jekel, Peter A., Baker, David, Marrink, Siewert J., Janssen, Dick B.

“…The ability to engineer enzymes and other proteins to any desired stability would have wide-ranging applications. Here, we demonstrate that computational…”
Get full text

A robust cosolvent-compatible halohydrin dehalogenase by computational library design by Arabnejad, Hesam, Dal Lago, Marco, Jekel, Peter A., Floor, Robert J., Thunnissen, Andy-Mark W. H., Terwisscha van Scheltinga, Anke C., Wijma, Hein J., Janssen, Dick B.

“…Abstract To improve the applicability of halohydrin dehalogenase as a catalyst for reactions in the presence of organic cosolvents, we explored a computational…”
Get full text

Biocatalytic and Structural Properties of a Highly Engineered Halohydrin Dehalogenase by Schallmey, Marcus, Floor, Robert J., Hauer, Bernhard, Breuer, Michael, Jekel, Peter A., Wijma, Hein J., Dijkstra, Bauke W., Janssen, Dick B.

“…Two highly engineered halohydrin dehalogenase variants were characterized in terms of their performance in dehalogenation and epoxide cyanolysis reactions…”
Get full text

X-ray crystallographic validation of structure predictions used in computational design for protein stabilization by Floor, Robert J., Wijma, Hein J., Jekel, Peter A., Terwisscha van Scheltinga, Anke C., Dijkstra, Bauke W., Janssen, Dick B.

“…ABSTRACT Protein engineering aimed at enhancing enzyme stability is increasingly supported by computational methods for calculation of mutant folding energies…”
Get full text

Enantioselective Enzymes by Computational Design and In Silico Screening by Wijma, Hein J., Floor, Robert J., Bjelic, Sinisa, Marrink, Siewert J., Baker, David, Janssen, Dick B.

“…Computational enzyme design holds great promise for providing new biocatalysts for synthetic chemistry. A strategy to design small mutant libraries of…”
Get full text

X-ray crystallographic validation of structure predictions used in computational design for protein stabilization: Comparing Predicted with Crystallographic Structures by Floor, Robert J., Wijma, Hein J., Jekel, Peter A., Terwisscha van Scheltinga, Anke C., Dijkstra, Bauke W., Janssen, Dick B.

Get full text