Search Results - "Fitchett, Jonathan R."

An anti-ANGPTL3/8 antibody decreases circulating triglycerides by binding to a LPL-inhibitory leucine zipper-like motif by Balasubramaniam, Deepa, Schroeder, Oliver, Russell, Anna M., Fitchett, Jonathan R., Austin, Aaron K., Beyer, Thomas P., Chen, Yan Q., Day, Jonathan W., Ehsani, Mariam, Heng, Aik Roy, Zhen, Eugene Y., Davies, Julian, Glaesner, Wolfgang, Jones, Bryan E., Siegel, Robert W., Qian, Yue-Wei, Konrad, Robert J.

“…Triglycerides (TG) are required for fatty acid transport and storage and are essential for human health. Angiopoietin-like-protein 8 (ANGPTL8) has previously…”
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Computationally Designed Bispecific Antibodies using Negative State Repertoires by Leaver-Fay, Andrew, Froning, Karen J., Atwell, Shane, Aldaz, Hector, Pustilnik, Anna, Lu, Frances, Huang, Flora, Yuan, Richard, Hassanali, Saleema, Chamberlain, Aaron K., Fitchett, Jonathan R., Demarest, Stephen J., Kuhlman, Brian

“…A challenge in the structure-based design of specificity is modeling the negative states, i.e., the complexes that you do not want to form. This is a difficult…”
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Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics by Froning, Karen, Maguire, Jack, Sereno, Arlene, Huang, Flora, Chang, Shawn, Weichert, Kenneth, Frommelt, Anton J., Dong, Jessica, Wu, Xiufeng, Austin, Heather, Conner, Elaine M., Fitchett, Jonathan R., Heng, Aik Roy, Balasubramaniam, Deepa, Hilgers, Mark T., Kuhlman, Brian, Demarest, Stephen J.

“…Recombinant T cell receptors (TCRs) can be used to redirect naïve T cells to eliminate virally infected or cancerous cells; however, they are plagued by low…”
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Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage by Pandya, Priyanka, Sayers, Robert O, Ting, Joey P, Morshedian, Shaghayegh, Torres, Carina, Cudal, Justine S, Zhang, Kai, Fitchett, Jonathan R, Zhang, Qing, Zhang, Feiyu F, Wang, Jing, Durbin, Jim D, Carrillo, Juan J, Espada, Alfonso, Broughton, Howard, Qian, Yuewei, Afshar, Sepideh

“…Hundreds of target specific peptides are routinely discovered by peptide display platforms. However, due to the high cost of peptide synthesis only a limited…”
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T cell receptor dwell times control the kinase activity of Zap70 by Klammt, Christian, Novotná, Lucie, Li, Dongyang T, Wolf, Miriam, Blount, Amy, Zhang, Kai, Fitchett, Jonathan R, Lillemeier, Björn F

“…Recruitment of the kinase Zap70 to the T cell receptor (TCR) is essential for signal transduction. Lillemeier and colleagues show that the conformation of…”
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An Automated Noncontact Deposition Interface for Liquid Chromatography Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry by Ericson, Christer, Phung, Qui T, Horn, David M, Peters, Eric C, Fitchett, Jonathan R, Ficarro, Scott B, Salomon, Arthur R, Brill, Laurence M, Brock, Ansgar

“…A new multichannel deposition system was developed for off-line liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry…”
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Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells by Zhang, Kai, Demarest, Stephen J, Wu, Xiufeng, Fitchett, Jonathan R

“…Glycoprofiling recombinant proteins expressed and secreted from mammalian cells is key to understanding their interactions with glycoprotein receptors in vivo…”
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Transient and stable CHO expression, purification and characterization of novel hetero‐dimeric bispecific IgG antibodies by Rajendra, Yashas, Peery, Robert B., Hougland, Maria D., Barnard, Gavin C., Wu, Xiufeng, Fitchett, Jonathan R., Bacica, Michael, Demarest, Stephen J.

“…IgG bispecific antibodies (BsAbs) represent one of the preferred formats for bispecific antibody therapeutics due to their native‐like IgG properties and their…”
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Fab-based bispecific antibody formats with robust biophysical properties and biological activity by Wu, Xiufeng, Sereno, Arlene J, Huang, Flora, Lewis, Steven M, Lieu, Ricky L, Weldon, Caroline, Torres, Carina, Fine, Cody, Batt, Micheal A, Fitchett, Jonathan R, Glasebrook, Andrew L, Kuhlman, Brian, Demarest, Stephen J

“…A myriad of innovative bispecific antibody (BsAb) platforms have been reported. Most require significant protein engineering to be viable from a development…”
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Protein design of IgG/TCR chimeras for the co-expression of Fab-like moieties within bispecific antibodies by Wu, Xiufeng, Sereno, Arlene J, Huang, Flora, Zhang, Kai, Batt, Micheal, Fitchett, Jonathan R, He, Dongmei, Rick, Heather L, Conner, Elaine M, Demarest, Stephen J

“…Immunoglobulins and T cell receptors (TCRs) share common sequences and structures. With the goal of creating novel bispecific antibodies (BsAbs), we generated…”
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T cell receptor dwell-times control the kinase activity of ZAP-70 by Klammt, Christian, Novotná, Lucie, Li, Dongyang T., Wolf, Miriam, Blount, Amy, Zhang, Kai, Fitchett, Jonathan R., Lillemeier, Björn F.

“…Kinase recruitment to membrane receptors is essential for signal transduction. However, the underlying regulatory mechanisms are poorly understood. We…”
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Transient and stable CHO expression, purification and characterization of novel hetero‐dimeric bispecific I g G antibodies by Rajendra, Yashas, Peery, Robert B., Hougland, Maria D., Barnard, Gavin C., Wu, Xiufeng, Fitchett, Jonathan R., Bacica, Michael, Demarest, Stephen J.

“…IgG bispecific antibodies (BsAbs) represent one of the preferred formats for bispecific antibody therapeutics due to their native‐like IgG properties and their…”
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Computationally Designed Bispecific Antibodies using Negative State Repertoires by Leaver-Fay, Andrew, Froning, Karen J., Atwell, Shane, Aldaz, Hector, Pustilnik, Anna, Lu, Frances, Huang, Flora, Yuan, Richard, Hassanali, Saleema, Chamberlain, Aaron K., Fitchett, Jonathan R., Demarest, Stephen J., Kuhlman, Brian

“…A challenge in the structure-based design of specificity is modeling the negative states, i.e., the complexes that you do not want to form. Here, this is a…”
Get full text