Search Results - "Fisher, Harvey F"

The application of transient-state kinetic isotope effects to the resolution of mechanisms of enzyme-catalyzed reactions by Fisher, Harvey F

“…Much of our understanding of the mechanisms of enzyme-catalyzed reactions is based on steady-state kinetic studies. Experimentally, this approach depends…”
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Approaches to the solution of coupled multiexponential transient-state rate kinetic equations: A critical review by Fisher, Harvey F.

“…The transient-state kinetic approach has failed to reach its full potential despite its advantage over the steady-state approach in its ability to observe…”
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Enzymatic reaction sequences as coupled multiple traces on a multidimensional landscape by Swint-Kruse, Liskin, Fisher, Harvey F.

“…We propose that an enzyme-catalyzed reaction is best described by a large, three-dimensional potential energy surface, defined by the number of enzyme…”
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Transient-State Kinetic Approach to Mechanisms of Enzymatic Catalysis by Fisher, Harvey F

“…Transient-state kinetics by its inherent nature can potentially provide more directly observed detailed resolution of discrete events in the mechanistic time…”
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Application of the Second Rule of Transient-State Kinetic Isotope Effects to an Enzymatic Mechanism by Fisher, Harvey F, Maniscalco, Steven J, Tally, Jon, Tabanor, Kayann

“…The transient-state kinetic approach reveals the formation and subsequent interconversions of intermediates in real time. Its potential for the mechanistic…”
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Detecting "silent" allosteric coupling by Fisher, Harvey F

“…Using isothermal calorimetry (ITC), we have found one case where a well-characterized allosteric activator showed no sign of allostery in its ΔG° of binding to…”
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Calorimetric methods for interpreting protein-ligand interactions by Fisher, H F, Singh, N

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Relationship between the Time-Dependence of a Transient-State Kinetic Isotope Effect and the Location of Complexes in a Reaction Sequence by Fisher, Harvey F, Palfey, Bruce A, Maniscalco, Steven J, Indyk, Lawrence

“…We prove here a new transient-state kinetic rule which states that the ratios of the first derivatives of kinetic isotope effect time courses, extrapolated to…”
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The interpretation of multiple-step transient-state kinetic isotope effects by Maniscalco, Steven J., Tally, Jon F., Fisher, Harvey F.

“…In contrast to steady-state kinetic isotope effects (KIEs), transient-state tKIEs are both time and signal dependent and therefore require a very different…”
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The direct measurement of thermodynamic parameters of reactive transient intermediates of the L-glutamate dehydrogenase reaction by Maniscalco, Steven J, Tally, Jon F, Harris, S Welsh, Fisher, Harvey F

“…In a previous report (Fisher, H. F., Maniscalco, S. J., and Tally, J. (2002) Biochem. Biophys. Res. Commun. 287, 343-347) we demonstrated the capability of the…”
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The Direct Measurement of Thermodynamic Parameters of Reactive Transient Intermediates of thel-Glutamate Dehydrogenase Reaction by Maniscalco, Steven J., Tally, Jon F., Harris, S. Welsh, Fisher, Harvey F.

“…In a previous report (Fisher, H. F., Maniscalco, S. J., and Tally, J. (2002) Biochem. Biophys. Res. Commun. 287, 343–347) we demonstrated the capability of the…”
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Detection of Multiple Active Site Domain Motions in Transient-State Component Time Courses of the Clostridium symbiosum l-Glutamate Dehydrogenase-Catalyzed Oxidative Deamination Reaction by Tally, Jon F, Maniscalco, Steven J, Saha, Swapan K, Fisher, Harvey F

“…We present a multiwavelength, transient-state kinetic study of the oxidative deamination reaction catalyzed by Clostridium symbiosum glutamate dehydrogenase…”
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Isoergonic Cooperativity in Glutamate Dehydrogenase Complexes:  A New Form of Allostery by Fisher, Harvey F, Tally, Jon

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A Close-Packed Planar 4-Atom Motif Serves as a Variable-Pathway Mechanistic Switching Device in Enzymatic Catalysis by Fisher, Harvey F., Maniscalco, Steven J.

“…We have found a new kind of structural motif that appears to be highly conserved among the pyridine nucleotide-linked α-amino acid dehydrogenases. This feature…”
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Identification and Characterization of Kinetically Competent Carbinolamine and α-Iminoglutarate Complexes in the Glutamate Dehydrogenase-Catalyzed Oxidation of l-Glutamate Using a Multiwavelength Transient State Approach by Maniscalco, Steven J, Saha, Swapan K, Fisher, Harvey F

“…A highly constrained and heavily overdetermined multiwavelength transient state kinetic approach has been used to study the oxidative deamination of…”
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Interpretation of Transient-State Kinetic Isotope Effects by Fisher, Harvey F, Saha, Swapan K

“…In contrast to steady-state kinetic isotope effects (KIE's), transient-state KIE's are dependent on both time and signal source. We developed a theory which…”
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Mechanistic Interpretation of Tryptophan Fluorescence Quenching in the Time Courses of Glutamate Dehydrogenase Catalyzed Reactions by Saha, Swapan K, Maniscalco, Steven J, Fisher, Harvey F

“…We have related the ratios of the protein fluorescence quenching and nucleotide absorbance time courses for the glutamate dehydrogenase catalyzed oxidative…”
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The pyridinium-dihydropyridine system. Reduction potentials and the mechanism of oxidation of 1,4-dihydropyridines by a Schiff base by Srinivasan, R, Medary, Richard T, Fisher, Harvey F, Norris, Donald J, Stewart, Ross

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A Difference in the Sequence of Steps in the Reactions Catalyzed by Two Closely Homologous Forms of Glutamate Dehydrogenase by Maniscalco, Steven J, Saha, Swapan K, Vicedomine, Patrick, Fisher, Harvey F

“…Glutamate dehydrogenase from beef liver (bl GDH) and the corresponding enzyme from Clostridium symbiosum (cs GDH) each catalyze the same sequence of chemical…”
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A Kinetic Mechanism of the Allosteric Control of Enzyme-Coenzyme Binding: Glutamate Dehydrogenase-NADPH-Phosphate-Acetate-Hydrogen Ion Interactions by Pazhanisamy, S, Maniscalco, Steven J, Singh, Narinder, Fisher, Harvey F

“…We have previously characterized the thermodynamic relationships which govern the dissociation of NADPH from bovine liver glutamate dehydrogenase and the…”
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