Search Results - "Fischer, Lutz"

Quirks of Error Estimation in Cross-Linking/Mass Spectrometry by Fischer, Lutz, Rappsilber, Juri

“…Cross-linking/mass spectrometry is an increasingly popular approach to obtain structural information on proteins and their complexes in solution. However,…”
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Rescuing error control in crosslinking mass spectrometry by Fischer, Lutz, Rappsilber, Juri

“…Crosslinking mass spectrometry is a powerful tool to study protein-protein interactions under native or near-native conditions in complex mixtures. Through…”
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Flavourzyme, an Enzyme Preparation with Industrial Relevance: Automated Nine-Step Purification and Partial Characterization of Eight Enzymes by Merz, Michael, Eisele, Thomas, Berends, Pieter, Appel, Daniel, Rabe, Swen, Blank, Imre, Stressler, Timo, Fischer, Lutz

“…Flavourzyme is sold as a peptidase preparation from Aspergillus oryzae. The enzyme preparation is widely and diversely used for protein hydrolysis in…”
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Defining the RNA interactome by total RNA‐associated protein purification by Shchepachev, Vadim, Bresson, Stefan, Spanos, Christos, Petfalski, Elisabeth, Fischer, Lutz, Rappsilber, Juri, Tollervey, David

“…The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)‐associated proteins. However, most cellular…”
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Microplastics detected in cirrhotic liver tissue by Horvatits, Thomas, Tamminga, Matthias, Liu, Beibei, Sebode, Marcial, Carambia, Antonella, Fischer, Lutz, Püschel, Klaus, Huber, Samuel, Fischer, Elke Kerstin

“…The contamination of ecosystem compartments by microplastics (MPs) is an ubiquitous problem. MPs have been observed in mice tissues, and recently in human…”
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Production Strategies and Applications of Microbial Single Cell Oils by Ochsenreither, Katrin, Glück, Claudia, Stressler, Timo, Fischer, Lutz, Syldatk, Christoph

“…Polyunsaturated fatty acids (PUFAs) of the ω-3 and ω-6 class (e.g., α-linolenic acid, linoleic acid) are essential for maintaining biofunctions in mammalians…”
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False discovery rate estimation and heterobifunctional cross-linkers by Fischer, Lutz, Rappsilber, Juri

“…False discovery rate (FDR) estimation is a cornerstone of proteomics that has recently been adapted to cross-linking/mass spectrometry. Here we demonstrate…”
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Secretion of the cytoplasmic and high molecular weight β-galactosidase of Paenibacillus wynnii with Bacillus subtilis by Senger, Jana, Seitl, Ines, Pross, Eva, Fischer, Lutz

“…The gram-positive bacterium Bacillus subtilis is widely used for industrial enzyme production. Its ability to secrete a wide range of enzymes into the…”
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Reliable identification of protein-protein interactions by crosslinking mass spectrometry by Lenz, Swantje, Sinn, Ludwig R., O’Reilly, Francis J., Fischer, Lutz, Wegner, Fritz, Rappsilber, Juri

“…Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the…”
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Protein complexes in cells by AI‐assisted structural proteomics by O'Reilly, Francis J, Graziadei, Andrea, Forbrig, Christian, Bremenkamp, Rica, Charles, Kristine, Lenz, Swantje, Elfmann, Christoph, Fischer, Lutz, Stülke, Jörg, Rappsilber, Juri

“…Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable…”
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Biotechnical production of trehalose through the trehalose synthase pathway: current status and future prospects by Cai, Xue, Seitl, Ines, Mu, Wanmeng, Zhang, Tao, Stressler, Timo, Fischer, Lutz, Jiang, Bo

“…Trehalose (α- d -glucopyranosyl-(1 → 1)-α- d -glucopyranoside) is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic…”
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Toward Oral Supplementation of Diamine Oxidase for the Treatment of Histamine Intolerance by Kettner, Lucas, Seitl, Ines, Fischer, Lutz

“…A new diamine oxidase (DAO-1) was discovered recently in the yeast Yarrowia lipolytica PO1f and investigated for its histamine degradation capability under…”
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The behavior of cathepsin D during milk processing and its contribution to bitterness in a model fresh cheese by Horstmann, Gudrun, Schäfer, Johannes, Rosenberger, Melanie, Seitl, Ines, Hinrichs, Jörg, Fischer, Lutz

“…The bovine endopeptidase cathepsin D was investigated regarding its temperature-dependent inactivation and ability to form bitter peptides within a spiked…”
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An integrated workflow for crosslinking mass spectrometry by Mendes, Marta L, Fischer, Lutz, Chen, Zhuo A, Barbon, Marta, O'Reilly, Francis J, Giese, Sven H, Bohlke‐Schneider, Michael, Belsom, Adam, Dau, Therese, Combe, Colin W, Graham, Martin, Eisele, Markus R, Baumeister, Wolfgang, Speck, Christian, Rappsilber, Juri

“…We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink…”
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Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica by Swietalski, Paul, Hetzel, Frank, Seitl, Ines, Fischer, Lutz

“…The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of…”
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Detection, production, and application of microbial arylsulfatases by Stressler, Timo, Seitl, Ines, Kuhn, Andreas, Fischer, Lutz

“…Arylsulfatases are enzymes which catalyze the hydrolysis of arylsulfate ester bonds to release a free sulfonate. They are widespread in nature and are found in…”
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Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii by Bechtel, Anna, Seitl, Ines, Pross, Eva, Hetzel, Frank, Keutgen, Mario, Fischer, Lutz

“…The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher…”
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Avoid “All-Touch” by Hybrid ALPPS to Achieve Oncological Efficacy by Li, Jun, Kantas, Alexandros, Ittrich, Harald, Koops, Andreas, Achilles, Eike G, Fischer, Lutz, Nashan, Bjoern

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Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry by Chen, Zhuo Angel, Jawhari, Anass, Fischer, Lutz, Buchen, Claudia, Tahir, Salman, Kamenski, Tomislav, Rasmussen, Morten, Lariviere, Laurent, Bukowski-Wills, Jimi-Carlo, Nilges, Michael, Cramer, Patrick, Rappsilber, Juri

“…Higher‐order multi‐protein complexes such as RNA polymerase II (Pol II) complexes with transcription initiation factors are often not amenable to X‐ray…”
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Computer-aided search for a cold-active cellobiose 2-epimerase by Chen, Qiuming, Xiao, Yaqin, Zhang, Wenli, Stressler, Timo, Fischer, Lutz, Jiang, Bo, Mu, Wanmeng

“…Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can catalyze bioconversion of lactose to its high-value derivatives, namely epilactose and…”
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