Biological Activity of Hybrid Vaterite-Pectin Microparticles Towards Bacteria E. coli and Human Neutrophils

Interaction of microbiota with hybrid vaterite-pectin microparticles as an attractive multifunctional vehicle for mucosal delivery should not provoke inflammation. Our purpose was to study the reaction of bacteria E. coli strain Mg1655 and isolate SharL from a patient with Crohn disease on the culti...

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Published in:	Bulletin of experimental biology and medicine Vol. 177; no. 2; pp. 238 - 242
Main Authors:	Galyamina, M. A., Pobeguts, O. V., Firova, R. K., Mosievich, D. V., Kharaeva, Z. F., Panasenko, O. M., Balabushevich, N. G., Mikhalchik, E. V.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-06-2024 Springer Nature B.V
Subjects:	Adenosine Triphosphate - metabolism Bacteria Biological activity Biomedical and Life Sciences Biomedicine Calcium Carbonate - chemistry Calcium Carbonate - pharmacology Cell activation Cell Biology Crohn Disease - microbiology Crohn Disease - pathology Crohn's disease Crohns disease E coli Escherichia coli - drug effects Humans Immunology and Microbiology Internal Medicine Intracellular Laboratory Medicine Leukocytes (neutrophilic) Microparticles Neutrophil Activation - drug effects Neutrophils Neutrophils - drug effects Neutrophils - metabolism Pathology Pectin Pectins - pharmacology Proteins hybrid microparticles vaterite-pectin; E. coli; neutrophils; ATP
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Summary:	Interaction of microbiota with hybrid vaterite-pectin microparticles as an attractive multifunctional vehicle for mucosal delivery should not provoke inflammation. Our purpose was to study the reaction of bacteria E. coli strain Mg1655 and isolate SharL from a patient with Crohn disease on the cultivation with hybrid microparticles and vaterite, and the subsequent activation of neutrophils. Vaterite-pectin microparticles enhanced leakage of ATP from bacteria. For E. coli Mg1655, the concentration of DNA decreased, while intracellular ATP increased. For E. coli SharL, the intracellular ATP decreased with simultaneous growth of DNA. Bacteria and microparticles together did not enhance activation of neutrophils in comparison with the particles per se in the medium without serum and in comparison with bacteria in the medium supplemented with serum; microparticles did not reduce functional activity of neutrophils.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0007-4888 1573-8221 1573-8221
DOI:	10.1007/s10517-024-06164-2