Potential biological control agents of Geosmithia morbida restrict fungal pathogen growth via mycoparasitism and antibiosis

Potential biological control agents of Geosmithia morbida restrict fungal pathogen growth via mycoparasitism and antibiosis

Thousand cankers disease of Juglans (walnut) and Pterocarya (wingnut) spp. (Fagales: Juglandaceae) is caused by the fungal pathogen Geosmithia morbida Kolarík, Freeland, Utley, and Tisserat (Hypocreales: Bionectriaceae) and bark beetle pest/vector, Pityophthorus juglandis Blackman (Coleoptera: Curcu...

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Published in:BioControl (Dordrecht, Netherlands) Vol. 69; no. 6; pp. 661 - 674
Main Authors: Onufrak, Aaron J., Gazis, Romina, Gwinn, Kimberly, Klingeman, William, Khodaei, Sima, Perez Oñate, Luis I., Finnell, Autumn, Givens, Spencer, Chen, Cristine, Holdridge, David R., Hadziabdic, Denita
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-12-2024
Springer Nature B.V
Subjects:
Agriculture
Animal Biochemistry
Animal Ecology
Antibiosis
Bark
Behavioral Sciences
Biological activity
Biological control
Biomedical and Life Sciences
Biotic factors
Disease control
Endophytes
Entomology
Entomopathogenic fungi
Fungi
Geosmithia morbida
Hypocreales
Juglans
Juglans spp
Juglone
Life Sciences
Metabolites
Mode of action
Pathogens
Phylogenetics
Pityophthorus juglandis
Plant Pathology
Pterocarya
thousand cankers disease
Trees
Trichoderma
Walnut
United States > US
spp
Thousand cankers disease
Juglone
Walnut
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Summary:Thousand cankers disease of Juglans (walnut) and Pterocarya (wingnut) spp. (Fagales: Juglandaceae) is caused by the fungal pathogen Geosmithia morbida Kolarík, Freeland, Utley, and Tisserat (Hypocreales: Bionectriaceae) and bark beetle pest/vector, Pityophthorus juglandis Blackman (Coleoptera: Curculionidae). To further the development of biological management strategies for thousand cankers disease, we assessed the ability of 14 endophytic Trichoderma (Hypocreales: Hypocreaceae) isolates and the commercially available isolate T. afroharzianum strain KRL-AG2 to inhibit the in vitro growth of three different G. morbida isolates via mycoparasitism and antibiosis. To identify factors that may affect field success of candidate biological control agents, we quantified the growth responses of Trichoderma spp. and the commercially available entomopathogenic fungus, Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) strain GHA, to the plant secondary metabolite and antimicrobial compound, juglone in vitro. A total of 12 Trichoderma isolates (from six different Trichoderma species) demonstrated antagonistic activity towards G. morbida in dual-plate assays. Juglone consistently reduced the growth of B. bassiana strain GHA and 14 of the 15 screened Trichoderma isolates in vitro. Additionally, one metabolite-producing Trichoderma isolate, TN4-47, completely inhibited the growth of all three G. morbida isolates across all tested metabolite concentrations and had comparatively greater tolerance to juglone compared to other Trichoderma isolates. Future lines of research should focus on characterizing the active antagonistic compound present in the metabolite filtrates, determine the mode of action of the active component(s), and elucidate how abiotic and biotic factors may influence the growth, persistence, and antagonistic activity of candidate biological control agents in planta .
Bibliography:USDA
AC05-00OR22725; 19-DG11083150-010
USDOE Office of Science (SC), Biological and Environmental Research (BER)
ISSN:1386-6141
1573-8248
DOI:10.1007/s10526-024-10277-y