High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria

High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria

microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to th...

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Bibliographic Details
Published in:Scientific reports Vol. 8; no. 1; p. 59
Main Authors: Yardeni, Tal, Fine, Raquel, Joshi, Yuvraj, Gradus-Pery, Tal, Kozer, Noga, Reichenstein, Irit, Yanowski, Eran, Nevo, Shir, Weiss-Tishler, Hila, Eisenberg-Bord, Michal, Shalit, Tal, Plotnikov, Alexander, Barr, Haim M., Perlson, Eran, Hornstein, Eran
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 08-01-2018
Nature Publishing Group
Subjects:
14/19
38/91
631/337/384/331
631/378/87
631/80
64/60
Humanities and Social Sciences
Image processing
Localization
miRNA
Mitochondria
Motor neurons
multidisciplinary
Neurodegenerative diseases
Rodents
Science
Science (multidisciplinary)
Vimentin
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Summary:microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the intermediate filament Vimentin is a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed intermediate filament network, upstream of mitochondria activity and cellular metabolism.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-17878-x