Search Results - "Findlay, Heather"

Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles by Varlas, Spyridon, Blackman, Lewis D, Findlay, Heather E, Reading, Eamonn, Booth, Paula J, Gibson, Matthew I, O’Reilly, Rachel K

“…Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of…”
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Detergent-free purification and reconstitution of functional human serotonin transporter (SERT) using diisobutylene maleic acid (DIBMA) copolymer by Dilworth, Marvin V, Findlay, Heather E, Booth, Paula J

“…Structure and function analysis of human membrane proteins in lipid bilayer environments is acutely lacking despite the fundame1ntal cellular importance of…”
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Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter by Jia, Ruyu, Martens, Chloe, Shekhar, Mrinal, Pant, Shashank, Pellowe, Grant A., Lau, Andy M., Findlay, Heather E., Harris, Nicola J., Tajkhorshid, Emad, Booth, Paula J., Politis, Argyris

“…Proton-coupled transporters use transmembrane proton gradients to power active transport of nutrients inside the cell. High-resolution structures often fail to…”
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Probing membrane protein properties using droplet interface bilayers by Allen-Benton, Maxwell, Findlay, Heather E, Booth, Paula J

“…Integral membrane proteins comprise a large proportion of drug targets, yet are challenging to study in vitro due to their amphiphilic nature. Conducting…”
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The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition by Findlay, Heather E., Booth, Paula J.

“…Lipids play key roles in Biology. Mechanical properties of the lipid bilayer influence their neighbouring membrane proteins, however it is unknown whether…”
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In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers by Findlay, Heather E., Harris, Nicola J., Booth, Paula J.

“…Nature encapsulates reactions within membrane-bound compartments, affording sequential and spatial control over biochemical reactions. Droplet Interface…”
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The membrane transporter lactose permease increases lipid bilayer bending rigidity by Lopez Mora, Nestor, Findlay, Heather E., Brooks, Nicholas J., Purushothaman, Sowmya, Ces, Oscar, Booth, Paula J.

“…Cellular life relies on membranes, which provide a resilient and adaptive cell boundary. Many essential processes depend upon the ease with which the membrane…”
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Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB by Reading, Eamonn, Ahdash, Zainab, Fais, Chiara, Ricci, Vito, Wang-Kan, Xuan, Grimsey, Elizabeth, Stone, Jack, Malloci, Giuliano, Lau, Andy M., Findlay, Heather, Konijnenberg, Albert, Booth, Paula J., Ruggerone, Paolo, Vargiu, Attilio V., Piddock, Laura J. V., Politis, Argyris

“…Resistance–nodulation–division efflux pumps play a key role in inherent and evolved multidrug resistance in bacteria. AcrB, a prototypical member of this…”
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Integrating Membrane Transporter Proteins into Droplet Interface Bilayers by Findlay, Heather E, Harris, Nicola J, Booth, Paula J

“…Droplet interface bilayers (DIBs) are an emerging tool within synthetic biology that aims to recreate biological processes in artificial cells. A critical…”
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Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies by Pellowe, Grant A, Findlay, Heather E, Lee, Karen, Gemeinhardt, Tim M, Blackholly, Laura R, Reading, Eamonn, Booth, Paula J

“…Co-translational folding studies of membrane proteins lag behind cytosolic protein investigations largely due to the technical difficulty in maintaining…”
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Methods to study folding of alpha-helical membrane proteins in lipids by Harris, Nicola J, Pellowe, Grant A, Blackholly, Laura R, Gulaidi-Breen, Samuel, Findlay, Heather E, Booth, Paula J

“…How alpha-helical membrane proteins fold correctly in the highly hydrophobic membrane interior is not well understood. Their folding is known to be highly…”
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Relative Domain Folding and Stability of a Membrane Transport Protein by Harris, Nicola J., Findlay, Heather E., Simms, John, Liu, Xia, Booth, Paula J.

“…There is a limited understanding of the folding of multidomain membrane proteins. Lactose permease (LacY) of Escherichia coli is an archetypal member of the…”
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Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein by Blackholly, Laura R, Harris, Nicola J, Findlay, Heather E, Booth, Paula J

“…The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies…”
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Light-activated control of protein channel assembly mediated by membrane mechanics by Miller, David M, Findlay, Heather E, Ces, Oscar, Templer, Richard H, Booth, Paula J

“…Photochemical processes provide versatile triggers of chemical reactions. Here, we use a photoactivated lipid switch to modulate the folding and assembly of a…”
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Conserved structure and domain organization among bacterial Slc26 transporters by Compton, Emma L R, Page, Kimberly, Findlay, Heather E, Haertlein, Michael, Moulin, Martine, Zachariae, Ulrich, Norman, David G, Gabel, Frank, Javelle, Arnaud

“…The Slc26 proteins are a ubiquitous superfamily of anion transporters conserved from bacteria to humans, among which four have been identified as human disease…”
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Unfolding free energy of a two-domain transmembrane sugar transport protein by Findlay, Heather E., Rutherford, Nicholas G., Henderson, Peter J. F., Booth, Paula J., Fersht, Alan R.

“…Understanding how an amino acid sequence folds into a functional, three-dimensional structure has proved to be a formidable challenge in biological research,…”
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Comparative stability of Major Facilitator Superfamily transport proteins by Harris, Nicola J., Findlay, Heather E., Sanders, Michael R., Kedzierski, Mateusz, dos Santos, Ália, Booth, Paula J.

“…Membrane transporters are a vital class of proteins for which there is little available structural and thermodynamic information. The Major Facilitator…”
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Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis Major Outer Membrane Protein by Findlay, Heather E, McClafferty, Heather, Ashley, Richard H

“…Chlamydial bacteria are obligate intracellular pathogens containing a cysteine-rich porin (Major Outer Membrane Protein, MOMP) with important structural and,…”
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Refinement of a morphological scoring system for postimplantation rabbit conceptuses by Carney, Edward W., Tornesi, Belen, Keller, Charles, Findlay, Heather A., Nowland, William S., Marshall, Valerie A., Ozolinš, Terence R.S.

“…BACKGROUND: The rabbit is used extensively in developmental toxicity testing, yet basic information on rabbit embryo development is lacking. The goals of this…”
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Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions by Reading, Eamonn, Hall, Zoe, Martens, Chloe, Haghighi, Tabasom, Findlay, Heather, Ahdash, Zainab, Politis, Argyris, Booth, Paula J.

“…The interplay between membrane proteins and the lipids of the membrane is important for cellular function, however, tools enabling the interrogation of protein…”
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