Covalent Fluorescence Labeling of His-Tagged Proteins on the Surface of Living Cells

Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐...

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Bibliographic Details
Published in:	Chembiochem : a European journal of chemical biology Vol. 9; no. 9; pp. 1391 - 1395
Main Authors:	Hintersteiner, Martin, Weidemann, Thomas, Kimmerlin, Thierry, Filiz, Nimet, Buehler, Christof, Auer, Manfred
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 16-06-2008 WILEY-VCH Verlag WILEY‐VCH Verlag
Subjects:	Cell Membrane - metabolism Cell Survival confocal fluorescence imaging Fluorescence Resonance Energy Transfer Fluorescent Dyes - metabolism Green Fluorescent Proteins - metabolism Histidine - metabolism Humans IL-4R Ni-NTA photoaffinity labeling Staining and Labeling - methods
Online Access:	Get full text
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Summary:	Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐tagged proteins on living cells was derived.
Bibliography:	http://dx.doi.org/10.1002/cbic.200800089 ark:/67375/WNG-8SWW38S2-Q ArticleID:CBIC200800089 istex:1421CE772841757E675D6724C28175AACE6BCCB7 These authors contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200800089