Cytotoxicity of submicron emulsions and solid lipid nanoparticles for dermal application

The cytotoxicity and physical properties of various submicron O/W emulsions and solid lipid nanoparticles for dermal applications were investigated. Droplet size and zetapotential of submicron emulsions depended on the composition of the cosurfactant blend used. The viability of J774 macrophages, mo...

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Bibliographic Details
Published in:	International journal of pharmaceutics Vol. 337; no. 1; pp. 291 - 298
Main Authors:	Weyenberg, Wim, Filev, Plamen, Van den Plas, Dave, Vandervoort, Jo, De Smet, Kris, Sollie, Philippe, Ludwig, Annick
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 07-06-2007 Elsevier
Subjects:	Administration, Cutaneous Amines - toxicity Animals BALB 3T3 Cells Biological and medical sciences Cell Survival - drug effects Cytotoxicity Drug Carriers Emulsions Fats - toxicity Fibroblasts - drug effects General pharmacology Humans Keratinocytes - drug effects Lipids - administration & dosage Lipids - chemistry Lipids - toxicity Macrophages - drug effects Medical sciences Mice Nanoparticles Particle Size Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Phosphatidylcholines - toxicity Phosphatidylserines - toxicity Polysorbates - toxicity Solid lipid nanoparticles Soybean Oil - toxicity Stearic Acids - toxicity Submicron emulsion Surface-Active Agents - administration & dosage Surface-Active Agents - chemistry Surface-Active Agents - toxicity Taurocholic Acid - toxicity Water - chemistry Zetapotential Cytotoxicity Submicron emulsion Zetapotential Particle size Solid lipid nanoparticles Emulsion Pharmaceutical technology Skin
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Summary:	The cytotoxicity and physical properties of various submicron O/W emulsions and solid lipid nanoparticles for dermal applications were investigated. Droplet size and zetapotential of submicron emulsions depended on the composition of the cosurfactant blend used. The viability of J774 macrophages, mouse 3T3 fibroblasts and HaCaT keratinocytes was significantly reduced in the presence of stearylamine. Nanoparticles consisting of stearic acid or different kinds of adeps solidus could be manufactured when formulated with lecithin, sodium taurocholate, polysorbate 80 and stearylamine. Survival of macrophages was highly affected by stearic acid and stearylamine. In general a viability of more than 90% was observed when semi-synthetic glycerides or hard fat was employed to formulate nanoparticles.
ISSN:	0378-5173 1873-3476
DOI:	10.1016/j.ijpharm.2006.12.045