Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research

The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis),...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of molecular sciences Vol. 25; no. 5; p. 2653
Main Authors:	Buzalewicz, Igor, Kaczorowska, Aleksandra, Fijałkowski, Wojciech, Pietrowska, Aleksandra, Matczuk, Anna Karolina, Podbielska, Halina, Wieliczko, Alina, Witkiewicz, Wojciech, Jędruchniewicz, Natalia
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 24-02-2024 MDPI
Subjects:	Anti-Bacterial Agents Antibiotics Bacteria bacterial biofilms Biofilms Cell death Cell division Contact lenses Contact Lenses, Hydrophilic - microbiology digital holographic tomography Drug resistance in microorganisms dynamics of biofilm formation Health aspects Humans Hydrogels Intraocular lenses Medical equipment Microscopy P. aeruginosa Performance evaluation Physiology Pseudomonas aeruginosa - physiology quantitative phase imaging S. epidermidis Tomography Poland Germany S. epidermidis soft contact lenses dynamics of biofilm formation quantitative phase imaging digital holographic tomography bacterial biofilms P. aeruginosa
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis), posing a significant risk to patients. Non-destructive and non-contact sensing techniques are essential in addressing this threat. Digital holographic tomography emerges as a promising solution. This allows for the 3D reconstruction of the refractive index distribution in biological samples, enabling label-free visualization and the quantitative analysis of biofilms. This tool provides insight into the dynamics of biofilm formation and maturation on the surface of transparent materials. Applying digital holographic tomography for biofilm examination has the potential to advance our ability to combat the antibiotic bacterial resistance crisis. A recent study focused on characterizing biofilm formation and maturation on six soft contact lens materials (three silicone hydrogels, three hydrogels), with a particular emphasis on and , both common culprits in ocular infections. The results revealed species- and time-dependent variations in the refractive indexes and volumes of biofilms, shedding light on cell dynamics, cell death, and contact lens material-related factors. The use of digital holographic tomography enables the quantitative analysis of biofilm dynamics, providing us with a better understanding and characterization of bacterial biofilms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms25052653