Clinical and genomic data of sars-cov-2 detected in maternal–fetal interface during the first wave of infection in Brazil

Brazil has the highest SARS-CoV-2 case-fatality rate in pregnant women in the Americas. In this study, clinical and virological findings of five mildly symptomatic pregnant women and their infected fetuses/newborns treated at a referral hospital for COVID19-pregnant women in Midwestern Brazil are re...

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Published in:	Microbes and infection Vol. 24; no. 4; p. 104949
Main Authors:	Ferreira, Maria de Fátima Carvalho, Pavon, Janeth Aracely Ramirez, Napoleão, Amanda Colichio Bini, Figueiredo, Gláucia Maria Duarte Preza, Florêncio, Patricia Cristina Borges, Arantes, Rayssa Basílio dos Santos, Rizzo, Paula Sossai, Carmo, Maria Aparecida Mazzutti Verlangieri, Nakazato, Luciano, Dutra, Valéria, Hahn, Rosane Christine, Slhessarenko, Renata Dezengrini
Format:	Journal Article
Language:	English
Published:	France Elsevier Masson SAS 01-06-2022 Published by Elsevier Masson SAS on behalf of Institut Pasteur
Subjects:	Clinical virology Coronavirus Molecular virology Neonatal infection Original Vertical transmission Molecular virology Vertical transmission Coronavirus Neonatal infection Clinical virology
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Summary:	Brazil has the highest SARS-CoV-2 case-fatality rate in pregnant women in the Americas. In this study, clinical and virological findings of five mildly symptomatic pregnant women and their infected fetuses/newborns treated at a referral hospital for COVID19-pregnant women in Midwestern Brazil are reported. Mother and fetal samples were tested by RT-qPCR, ECLIA and Illumina MiSeq sequencing. From the five cases, one resulted in spontaneous abortion, one was stillborn, two were preterm births and one full-term birth. Maternal and fetal placenta, newborn and stillborn secretions were SARS-CoV-2+; one neonate developed ground-glass opacities in his lungs. One neonate's umbilical cord was IgG+ and all were IgM negative upon hospital discharge. Genomes recovered from two placentas belong to the B.1.1.28 and B.1.1.33 lineages and present nonsynonymous mutations associated with virus fitness and infectivity; other not frequently reported mutations (B.1.1.33: NSP3 V2090G, M A2S and ORF3ab S253P and Y264N; B.1.1.28: NSP3 E995D, NSP12 R240K, NSP14H1897Y and in ORF7b V21F) were found in proteins involved in viral replication, viral induction of apoptosis, viral interference on interferon and on NF-Κβ pathways. Phylogeny indicates the south of Brazil as the possible origin of these lineages circulating in Mato Grosso State. These findings contribute to describe SARS-CoV-2 infection and outcomes in pregnant women and their fetuses, at any stage of gestation and even in mild symptomatic cases.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1286-4579 1769-714X
DOI:	10.1016/j.micinf.2022.104949