Search Results - "Fierer, Jacob"
-
1
SpyLigase peptide–peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture
Published in Proceedings of the National Academy of Sciences - PNAS (01-04-2014)“…Individual proteins can now often be modified with atomic precision, but there are still major obstacles to connecting proteins into larger assemblies. To…”
Get full text
Journal Article -
2
Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag
Published in Journal of molecular biology (23-01-2014)“…Peptide tagging is a key strategy for observing and isolating proteins. However, the interactions of proteins with peptides are nearly all rapidly reversible…”
Get full text
Journal Article -
3
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
Published in Proceedings of the National Academy of Sciences - PNAS (20-03-2012)“…Protein interactions with peptides generally have low thermodynamic and mechanical stability. Streptococcus pyogenes fibronectin-binding protein FbaB contains…”
Get full text
Journal Article -
4
SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme
Published in Angewandte Chemie International Edition (10-06-2014)“…SpyTag is a peptide that spontaneously forms an amide bond with its protein partner SpyCatcher. SpyTag was fused at the N terminus of β‐lactamase and…”
Get full text
Journal Article -
5
SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme
Published in Angewandte Chemie (10-06-2014)“…SpyTag is a peptide that spontaneously forms an amide bond with its protein partner SpyCatcher. SpyTag was fused at the N terminus of β‐lactamase and…”
Get full text
Journal Article -
6
SpyLigase peptide—peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture
Published in Proceedings of the National Academy of Sciences - PNAS (01-04-2014)Get full text
Journal Article -
7
Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences
Published in Cell discovery (09-06-2015)“…The Dnmt2 RNA methyltransferase catalyses the methylation of C38 in the anticodon loop of tRNA-Asp, but the molecular role of this methylation is unknown…”
Get full text
Journal Article -
8
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
Published in Proceedings of the National Academy of Sciences - PNAS (20-03-2012)Get full text
Journal Article