Search Results - "Fieder, Juergen"

Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing by Stadermann, Anna, Gamer, Martin, Fieder, Jürgen, Lindner, Benjamin, Fehrmann, Steffen, Schmidt, Moritz, Schulz, Patrick, Gorr, Ingo H.

“…Genetically modified CHO cell lines are traditionally used for the production of biopharmaceuticals. However, an in‐depth molecular understanding of the…”
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miRNA engineering of CHO cells facilitates production of difficult‐to‐express proteins and increases success in cell line development by Fischer, Simon, Marquart, Kim F., Pieper, Lisa A., Fieder, Juergen, Gamer, Martin, Gorr, Ingo, Schulz, Patrick, Bradl, Harald

“…ABSTRACT In recent years, coherent with growing biologics portfolios also the number of complex and thus difficult‐to‐express (DTE) therapeutic proteins has…”
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Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy by Mathias, Sven, Fischer, Simon, Handrick, René, Fieder, Jürgen, Schulz, Patrick, Bradl, Harald, Gorr, Ingo, Gamer, Martin, Otte, Kerstin

“…[Display omitted] •A method examining a recombinant proteins intracellular distribution is presented.•For a bispecific antibody the ER was shown to be the most…”
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Towards maximum acceleration of monoclonal antibody development: Leveraging transposase-mediated cell line generation to enable GMP manufacturing within 3 months using a stable pool by Schmieder, Valerie, Fieder, Juergen, Drerup, Raphael, Gutierrez, Erik Arango, Guelch, Carina, Stolzenberger, Jessica, Stumbaum, Mihaela, Mueller, Volker Steffen, Higel, Fabian, Bergbauer, Martin, Bornhoefft, Kim, Wittner, Manuel, Gronemeyer, Petra, Braig, Christian, Huber, Michaela, Reisenauer-Schaupp, Anita, Mueller, Markus Michael, Schuette, Mark, Puengel, Sebastian, Lindner, Benjamin, Schmidt, Moritz, Schulz, Patrick, Fischer, Simon

“…In recent years, acceleration of development timelines has become a major focus within the biopharmaceutical industry to bring innovative therapies faster to…”
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A single‐step FACS sorting strategy in conjunction with fluorescent vital dye imaging efficiently assures clonality of biopharmaceutical production cell lines by Fieder, Jürgen, Schulz, Patrick, Gorr, Ingo, Bradl, Harald, Wenger, Till

“…The development of biopharmaceutical production cell lines typically starts with generation of heterogeneous populations of cells, from which then single cell…”
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Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells by Fieder, Jürgen, Wagner, Nina, Grammatikos, Stefanos, Hoffmann, Helmut, Kaufmann, Hitto, Otto, Ralf T.

“…Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP…”
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