Quantitative phase imaging for characterization of single cell growth dynamics

Quantitative phase imaging for characterization of single cell growth dynamics

Quantitative phase imaging (QPI) has emerged as an indispensable tool in the field of biomedicine, offering the ability to obtain quantitative maps of phase changes due to optical path length delays without the need for contrast agents. These maps provide valuable information about cellular morpholo...

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Bibliographic Details
Published in:Lasers in medical science Vol. 38; no. 1; p. 241
Main Authors: Butt, Summiah Saleem, Fida, Irum, Fatima, Maham, Khan, Muskan Saif, Mustafa, Sonia, Khan, Muhammad Noman, Ahmad, Iftikhar
Format: Journal Article
Language:English
Published: London Springer London 18-10-2023
Springer Nature B.V
Subjects:
Apoptosis
Biomedical research
Cell Death
Cell growth
Cell Proliferation
Contrast agents
Contrast media
Dentistry
Interferometry
Lasers
Medical research
Medicine
Medicine & Public Health
Microscopy
Mitosis
Morphology
Necrosis
Optical Devices
Optics
Organelles
Photonics
Quantum Optics
Review Article
Optical phase
Refractive index
Fluorescence microscopy
Interference
Apoptosis
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Summary:Quantitative phase imaging (QPI) has emerged as an indispensable tool in the field of biomedicine, offering the ability to obtain quantitative maps of phase changes due to optical path length delays without the need for contrast agents. These maps provide valuable information about cellular morphology and dynamics, unperturbed by the introduction of exogenous substances. In this review, a summary of recent studies that have focused on elucidating the growth dynamics of individual cells using QPI is presented. Specifically, investigations into cellular changes occurring during mitosis, the differentiation of cellular organelles, the assessment of distinct cell death processes (i.e., apoptosis, necrosis, and oncosis) and the precise measurement of live cell temperature are explored. Furthermore, the captivating applications of QPI in theragnostics, where its potential for transformative impact is prominently showcased, are highlighted. Finally, the challenges that need to be overcome for its wider adoption and successful integration into biomedical research are outlined. Graphical abstract
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ObjectType-Review-1
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-023-03902-2