A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

Heterozygous mutations in the TBK1 gene can cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The majority of TBK1-ALS/FTD patients carry deleterious loss-of-expression mutations, and it is still unclear which TBK1 function leads to neurodegeneration. We investigated the i...

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Published in:	The Journal of experimental medicine Vol. 221; no. 5
Main Authors:	Brenner, David, Sieverding, Kirsten, Srinidhi, Jahnavi, Zellner, Susanne, Secker, Christopher, Yilmaz, Rüstem, Dyckow, Julia, Amr, Shady, Ponomarenko, Anna, Tunaboylu, Esra, Douahem, Yasmin, Schlag, Joana S, Rodríguez Martínez, Lucía, Kislinger, Georg, Niemann, Cornelia, Nalbach, Karsten, Ruf, Wolfgang P, Uhl, Jonathan, Hollenbeck, Johanna, Schirmer, Lucas, Catanese, Alberto, Lobsiger, Christian S, Danzer, Karin M, Yilmazer-Hanke, Deniz, Münch, Christian, Koch, Philipp, Freischmidt, Axel, Fetting, Martina, Behrends, Christian, Parlato, Rosanna, Weishaupt, Jochen H
Format:	Journal Article
Language:	English
Published:	United States 06-05-2024
Online Access:	Get full text
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Summary:	Heterozygous mutations in the TBK1 gene can cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The majority of TBK1-ALS/FTD patients carry deleterious loss-of-expression mutations, and it is still unclear which TBK1 function leads to neurodegeneration. We investigated the impact of the pathogenic TBK1 missense variant p.E696K, which does not abolish protein expression, but leads to a selective loss of TBK1 binding to the autophagy adaptor protein and TBK1 substrate optineurin. Using organelle-specific proteomics, we found that in a knock-in mouse model and human iPSC-derived motor neurons, the p.E696K mutation causes presymptomatic onset of autophagolysosomal dysfunction in neurons precipitating the accumulation of damaged lysosomes. This is followed by a progressive, age-dependent motor neuron disease. Contrary to the phenotype of mice with full Tbk1 knock-out, RIPK/TNF-α-dependent hepatic, neuronal necroptosis, and overt autoinflammation were not detected. Our in vivo results indicate autophagolysosomal dysfunction as a trigger for neurodegeneration and a promising therapeutic target in TBK1-ALS/FTD.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1007 1540-9538
DOI:	10.1084/jem.20221190