Effects of chlorophyllin on replication of poliovirus and bovine herpesvirus in vitro

Effects of chlorophyllin on replication of poliovirus and bovine herpesvirus in vitro

Chlorophyllin (CHLN), a synthetic derivative of chlorophyll, was assayed in the replication of poliovirus (PV-1) and bovine herpesvirus (BoHV-1) in HEp-2 cell cultures. Virucidal activity of CHLN was evaluated and the time-of-addition assay was performed as follows: before the infection (-1 and -2 h...

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Bibliographic Details
Published in:Letters in applied microbiology Vol. 49; no. 6; pp. 791 - 795
Main Authors: Benati, F.J, Lauretti, F, Faccin, L.C, Nodari, B, Ferri, D.V, Mantovani, M.S, Linhares, R.E.C, Nozawa, C
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-12-2009
Blackwell Publishing Ltd
Blackwell
Subjects:
antiviral
Antiviral Agents - pharmacology
Biological and medical sciences
bovine herpesvirus
Cell Line, Tumor
Chlorophyllides - pharmacology
chlorophyllin
Cytopathogenic Effect, Viral - drug effects
Fundamental and applied biological sciences. Psychology
Herpesviridae - drug effects
Herpesviridae - physiology
Humans
Inhibitory Concentration 50
Microbiology
plaque
Poliovirus
Poliovirus - drug effects
Poliovirus - physiology
Viral Plaque Assay
Virus Replication - drug effects
Virus
chlorophyllin
plaque
Picornaviridae
Applied microbiology
Herpesviridae
Bovine herpesvirus
Antiviral
Replication
Enterovirus
Poliovirus
In vitro
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Summary:Chlorophyllin (CHLN), a synthetic derivative of chlorophyll, was assayed in the replication of poliovirus (PV-1) and bovine herpesvirus (BoHV-1) in HEp-2 cell cultures. Virucidal activity of CHLN was evaluated and the time-of-addition assay was performed as follows: before the infection (-1 and -2 h), at the time of the infection (0 h) and after the infection (1 and 2 h). Plaque reduction assay (PRA) showed that CHLN inhibited BoHV-1 and PV-1 infection and the 50% inhibitory concentrations (IC₅₀) against BoHV-1 and PV-1 infection were 8·6 and 19·8 μg ml⁻¹, respectively. The time-of-addition study demonstrated that the CHLN was effective inhibiting viral replication in 51% and 66·5% for PV-1 and BoHV-1, respectively, at the highest concentration of 20·0 μg ml⁻¹, when added during the infection. The directed effect of CHLN on viral strains demonstrated an inhibition of 62% and 66·4% for PV-1 and BoHV-1, respectively, by PRA. These results demonstrated that CHLN could be used as an antiviral suggesting directed activity on virus particles and on virus-receptor sites to BoHV. For poliovirus, CHLN also demonstrated virucide activity, moreover, showed to inhibit early steps of the replication cycle. CHLN demonstrated promising selectivity index for both virus strains; therefore, it can be used for the development of an antiviral agent.
Bibliography:http://dx.doi.org/10.1111/j.1472-765X.2009.02744.x
ObjectType-Article-1
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ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.2009.02744.x