GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil (EOOO) and safrole agai...

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Published in:Antibiotics (Basel) Vol. 9; no. 5; p. 247
Main Authors: Almeida, Ray S, Freitas, Priscilla R, Araújo, Ana Carolina J, Menezes, Irwin R A, Santos, Eduardo L, Tintino, Saulo R, Moura, Talysson F, Filho, Jaime R, Ferreira, Vitória A, Silva, Ana Cristina A, Silva, Luiz E, Amaral, Wanderlei do, Deschamps, Cícero, Iriti, Marcello, Coutinho, Henrique D M
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 12-05-2020
MDPI
Subjects:
bacterial resistance
biological activity
chemical composition
Essences and essential oils
ethidium bromide
Gas chromatography
Health aspects
Laurel
Mass spectrometry
Methods
Prevention
Staphylococcus aureus
Brazil
biological activity
chemical composition
bacterial resistance
ethidium bromide
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Summary:Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of and were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in aureus. In conclusion, and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.
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ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9050247