Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri

The genome of Xanthomonas citri subsp. Citri strain 306 pathotype A ( Xac ) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the...

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Published in:	Functional & integrative genomics Vol. 15; no. 2; pp. 197 - 210
Main Authors:	Moreira, Leandro Marcio, Facincani, Agda Paula, Ferreira, Cristiano Barbalho, Ferreira, Rafael Marine, Ferro, Maria Inês Tiraboshi, Gozzo, Fabio Cesar, de Oliveira, Julio Cezar Franco, Ferro, Jesus Aparecido, Soares, Márcia Regina
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2015 Springer Nature B.V
Subjects:	Adaptation, Biological Animal Genetics and Genomics Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Sites Biochemistry Bioinformatics Biomedical and Life Sciences Cell Biology Chemotaxis Citrus Citrus - microbiology Flagella - physiology Genomics Life Sciences Metabolism Microbial Genetics and Genomics Mutation Original Paper Phosphates Phosphates - metabolism Plant Diseases - microbiology Plant Genetics and Genomics Regulon Signal Transduction Transcription Factors - metabolism Xanthomonas Xanthomonas - genetics Xanthomonas - metabolism Xanthomonas - pathogenicity Xanthomonas - physiology adaptation Two-component systems PhoBR and PhoPQ Phosphate metabolism Chemotaxis signal transduction Virulence
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Summary:	The genome of Xanthomonas citri subsp. Citri strain 306 pathotype A ( Xac ) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the plant. Proteomic analysis of Xac showed that the processes of chemotactic signal transduction and phosphate metabolism are key adaptive strategies during the interaction of a pathogenic bacterium with its plant host. The results also indicate the importance of a group of proteins that may not be directly related to the classical virulence factors, but that are likely fundamental to the success of the initial stages of the infection, such as methyl-accepting chemotaxis protein (Mcp) and phosphate specific transport (Pst). Furthermore, the analysis of the mutant of the gene pstB which codifies to an ABC phosphate transporter subunit revealed a complete absence of citrus canker symptoms when inoculated in compatible hosts. We also conducted an in silico analysis which established the possible network of genes regulated by two-component systems PhoPQ and PhoBR (related to phosphate metabolism), and possible transcriptional factor binding site (TFBS) motifs of regulatory proteins PhoB and PhoP, detaching high degree of conservation of PhoB TFBS in 84 genes of Xac genome. This is the first time that chemotaxis signal transduction and phosphate metabolism were therefore indicated to be fundamental to the process of colonization of plant tissue during the induction of disease associated with Xanthomonas genus bacteria.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1438-793X 1438-7948
DOI:	10.1007/s10142-014-0414-z