Inhibitory effects of a standardized extract of Justicia pectoralis in an experimental rat model of airway hyper‐responsiveness

Inhibitory effects of a standardized extract of Justicia pectoralis in an experimental rat model of airway hyper‐responsiveness

Objective Justicia pectoralis is a plant useful for the treatment of respiratory diseases. Here, we studied the antiasthmatic properties of a standardized extract of J. pectoralis (Jp). Methods Ovalbumin (OVA)‐sensitized rats were actively challenged with saline or OVA to study airway hyper‐responsi...

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Bibliographic Details
Published in:Journal of pharmacy and pharmacology Vol. 69; no. 6; pp. 722 - 732
Main Authors: Moura, Carlos T. M., Batista‐Lima, Francisco J., Brito, Teresinha S., Silva, Alfredo A. V., Ferreira, Luan C., Roque, Cássia R., Aragão, Karoline S., Havt, Alexandre, Fonseca, Francisco N., Leal, Luzia K. A. M., Magalhães, Pedro J. C.
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-06-2017
Subjects:
Acetylcholine
Alveoli
Animals
Asthma - chemically induced
Asthma - drug therapy
Asthma - metabolism
biological evaluation of natural products
Bronchoalveolar Lavage Fluid - chemistry
Bronchus
Calcium
Calcium - metabolism
Calcium influx
Carbachol
Contractility
Contraction
Coumarin
Gene expression
Interleukin 1
Justicia - chemistry
Male
Models, Animal
Ovalbumin
Ovalbumin - pharmacology
Plant Extracts - pharmacology
Potassium chloride
Proteins
Rats
Rats, Wistar
Respiratory diseases
Respiratory Hypersensitivity - chemically induced
Respiratory Hypersensitivity - drug therapy
Respiratory Hypersensitivity - metabolism
Respiratory tract
Rodents
Stores
Thapsigargin
tissue and cellular pharmacology
Tissues
Trachea
Trachea - drug effects
Trachea - metabolism
Tumor Necrosis Factor-alpha - metabolism
tissue and cellular pharmacology
biological evaluation of natural products
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Summary:Objective Justicia pectoralis is a plant useful for the treatment of respiratory diseases. Here, we studied the antiasthmatic properties of a standardized extract of J. pectoralis (Jp). Methods Ovalbumin (OVA)‐sensitized rats were actively challenged with saline or OVA to study airway hyper‐responsiveness after oral treatment with saline or Jp. The ability of Jp to inhibit hyper‐reactivity was evaluated in isolated trachea mounted in isolated organ bath chamber. Key findings Using KCl or carbachol as contractile agents, tracheal rings of OVA‐challenged rats contracted with higher magnitude than trachea of rats challenged with saline. Such hyper‐responsive phenotype of OVA‐challenged tissues decreased with Jp administration. In Ca+‐free medium, Jp or its major constituent coumarin inhibited preferentially the contractions induced by Ca2+ addition in tissues of OVA‐challenged rats stimulated with KCl or acetylcholine. In tissues depleted of their internal Ca+ stores in the presence of thapsigargin, Jp inhibited the contraction induced by capacitative Ca2+ entry. By gavage, Jp abolished the increase caused by challenge with OVA on the levels of IL‐1β and TNF‐α in the bronchoalveolar fluid and also impaired the changes in gene expression of canonical transient receptor proteins. Conclusions Jp has antiasthmatic properties in an experimental model that reproduces tracheal hyper‐reactivity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-3573
2042-7158
DOI:10.1111/jphp.12689