Chemical and genetic characterization of lipopeptides from Bacillus velezensis and Paenibacillus ottowii with activity against Fusarium verticillioides
The fungus significantly threatens maize crops in tropical soils. In light of this, biological control has emerged as a promising strategy to reduce fungicide costs and environmental risks. In this study, we aimed to test the antifungal activity of cell-free supernatant (CFS) from three (CT02, IM14,...
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| Published in: | Frontiers in microbiology Vol. 15; p. 1443327 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
26-08-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The fungus
significantly threatens maize crops in tropical soils. In light of this, biological control has emerged as a promising strategy to reduce fungicide costs and environmental risks. In this study, we aimed to test the antifungal activity of cell-free supernatant (CFS) from three
(CT02, IM14, and LIS05) and one
(LIS04) against
, thereby contributing to the development of effective biocontrol measures.
The research employed a comprehensive approach. The antifungal activity of the bacterial strains was tested using cell-free supernatant (CFS) from three
(CT02, IM14, and LIS05) and one
(LIS04). The UPLC-MS evaluated the CFS to identify the main bioactive molecules involved in the inhibitory effect on
.
. Scanning electron microscopy (SEM) was used to assess the impact of CFS on spores and hyphae, and genome sequencing was conducted to identify the genes involved in biological control. These robust methodologies ensure the reliability and validate our findings.
The CFS of the four strains demonstrated significant inhibition of fungal growth. The UPLC-MS analysis revealed the presence of lipopeptides with antifungal activity, including surfactin and fengycins A and B expressed by the three strains of
and iturin A expressed by strains LIS05 and IM14. For
, fusaricidins, ABCDE, and five previously unreported lipopeptides were detected. Scanning electron microscopy (SEM) showed that treatments with CFS led to significant distortion and breakage of the
.
hyphae, in addition to the formation of cavities in the membrane. Genome mining confirmed the presence of genes coding for the lipopeptides identified by UPLC-MS, including the gene for iturin in CTO2. Genomic sequencing revealed that CT02, IM14, and LIS05 belong to different strains of
, and LIS04 belongs to
, a species recently described.
The four bacterial strains, including three novel strains identified as
and one as the recently described species
, demonstrate significant potential as biocontrol agents for managing fungal disease. This finding underscores the novelty and potential impact of our research. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Vera Lúcia dos Santos orcid.org/0000-0001-5815-5652 José Edson Fontes Figueiredo orcid.org/0000-0002-9542-2315 Kirley Marques Canuto orcid.org/0000-0003-3194-6125 ORCID: Gisele de Fátima Dias Diniz orcid.org/0000-0001-7018-6574 Christiane Abreu de Oliveira-Paiva orcid.org/0000-0002-1874-2489 Ana Sheila de Queiroz Souza orcid.org/0000-0002-1536-6588 Maria Lúcia Ferreira Simeone orcid.org/0000-0002-2003-0341 Sylvia Morais de Sousa Tinoco orcid.org/0000-0002-8030-1385 Reviewed by: Ravindra Soni, Indira Gandhi Krishi Vishva Vidyalaya, India Mehi Lal, ICAR-Central Potato Research Institute, India Mikaely Sousa Marins orcid.org/0000-0002-3508-8204 Luciano Viana Cota orcid.org/0000-0003-4216-7610 Paulo Riceli Vasconcelos Ribeiro orcid.org/0000-0002-5258-4015 Edited by: Vinay Kumar, ICAR-National Institute of Biotic Stress Management, India Lourenço Vitor Silva Ferreira orcid.org/0009-0003-8797-5213 |
| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2024.1443327 |