Th1/Th2/Th17/Treg Balance in Apical Periodontitis of Normoglycemic and Diabetic Rats

Th1/Th2/Th17/Treg Balance in Apical Periodontitis of Normoglycemic and Diabetic Rats

The aim of this study was to evaluate the inflammatory profile of T helper (Th) cells in normoglycemic (N) and diabetic rats with apical periodontitis (AP). Twenty male Wistar rats were divided in 2 groups: N rats and rats with diabetes mellitus (DM). DM was induced using streptozotocin, and AP was...

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Bibliographic Details
Published in:Journal of endodontics Vol. 45; no. 8; pp. 1009 - 1015
Main Authors: Samuel, Renata Oliveira, Ervolino, Edilson, de Azevedo Queiroz, Índia Olinta, Azuma, Mariane Maffei, Ferreira, Gabriela Tiago, Cintra, Luciano Tavares Angelo
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-08-2019
Subjects:
Animals
Apical periodontitis
Dentistry
diabetes mellitus
Diabetes Mellitus, Experimental
Humans
Inflammation
Male
Periapical Periodontitis - metabolism
Rats
Rats, Wistar
T-Lymphocytes, Regulatory
Th1 Cells
Th17 Cells
Th2 Cells
inflammation
diabetes mellitus
Apical periodontitis
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Summary:The aim of this study was to evaluate the inflammatory profile of T helper (Th) cells in normoglycemic (N) and diabetic rats with apical periodontitis (AP). Twenty male Wistar rats were divided in 2 groups: N rats and rats with diabetes mellitus (DM). DM was induced using streptozotocin, and AP was induced by dental pulp exposure of the first mandibular molar to the oral environment. After 30 days, the mandibles were removed and processed for histologic analysis, bacterial analysis, and immunochemical assays for interleukin (IL)-6, tumor necrosis factor alpha, IL-17, IL-23, interferon gamma, and IL-10. The Mann-Whitney U test and Student t test were used for statistical analysis (P < .05). The DM group showed more intense inflammatory infiltrate with larger sizes of bone reabsorption and a greater presence of bacteria than the N group (P < .05). Proinflammatory cytokine levels in the DM group were also greater than those in the N group (P < .05). However, interferon gamma was more intense in the N group than in the DM group (P < .05). The inflammatory profile of AP in DM is different from that in the N group, suggesting that Th1 is a secondary strain and the Th17 strain is predominant in DM. •The hyperglycemic condition seems to affect the immune response profile in apical periodontitis pathogenesis.•The inflammatory profile of apical periodontitis in the diabetic condition is different from that in the normoglycemic condition.•The Th17 pathway is predominant in diabetic individuals, whereas the Th1 pathway is shown to be secondary.
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ISSN:0099-2399
1878-3554
DOI:10.1016/j.joen.2019.05.003