Prolactin-induced changes in protein expression in human pancreatic islets

Ex vivo islet cell culture prior to transplantation appears as an attractive alternative for treatment of type 1 diabetes. Previous results from our laboratory have demonstrated beneficial effects of human prolactin (rhPRL) treatment on human islet primary cultures. In order to probe into the molecu...

Full description

Saved in:

Bibliographic Details
Published in:	Molecular and cellular endocrinology Vol. 264; no. 1; pp. 16 - 27
Main Authors:	Labriola, L., Ferreira, G. Bomfim, Montor, W.R., Demasi, M.A.A., Pimenta, D.C., Lojudice, F.H., Genzini, T., Goldberg, A.C., Eliaschewitz, F.G., Sogayar, M.C.
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 29-01-2007
Subjects:	Adult Cell Proliferation - drug effects Diabetes Electrophoresis, Gel, Two-Dimensional Female Gene Expression Regulation - drug effects Human islets Humans Insulin Islets of Langerhans - cytology Islets of Langerhans - metabolism Islets of Langerhans Transplantation Male Mass Spectrometry Middle Aged Prolactin Prolactin - pharmacology Proteomics Recombinant Fusion Proteins - pharmacology Tissue Culture Techniques Human islets Diabetes Prolactin Insulin Proteomics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Ex vivo islet cell culture prior to transplantation appears as an attractive alternative for treatment of type 1 diabetes. Previous results from our laboratory have demonstrated beneficial effects of human prolactin (rhPRL) treatment on human islet primary cultures. In order to probe into the molecular events involved in the intracellular action of rhPRL in these cells, we set out to identify proteins with altered expression levels upon rhPRL cell treatment, using two-dimensional (2D) gel electrophoresis and mass spectrometry (MS). An average of 300 different protein spots were detected, 14 of which were modified upon rhPRL treatment ( p < 0.01), of which 12 were successfully identified using MS and grouped according to their biological functions. In conclusion, our study provides, for the first time, information about proteins that could be critically involved in PRL's action on human pancreatic islets, and facilitate identification of new and specific targets involved in islet cell function and proliferation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0303-7207 1872-8057
DOI:	10.1016/j.mce.2006.10.004