Adjusted method of penis fixation during boar semi‐automatic semen collection aiming to reduce bacterial contamination

Adjusted method of penis fixation during boar semi‐automatic semen collection aiming to reduce bacterial contamination

Semen collection has an essential role in the initial bacterial load in boar ejaculates and extended semen. The study aimed to explore the efficacy of an adjusted penis fixation in a semi‐automatic collection system on reducing bacterial contamination of ejaculates in two‐boar studs with different s...

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Bibliographic Details
Published in:Reproduction in domestic animals Vol. 56; no. 6; pp. 897 - 904
Main Authors: Paschoal, Aline Fernanda Lopes, Mellagi, Ana Paula Gonçalves, Ferrari, Cristina Vicenti, Takeuti, Karine Ludwig, Oliveira, Gabriela da Silva, Bernardi, Mari Lourdes, Ulguim, Rafael da Rosa, Bortolozzo, Fernando Pandolfo
Format: Journal Article
Language:English
Published: Germany Blackwell Publishing Ltd 01-06-2021
Subjects:
aerobic mesophiles
Bacteria
bacterial load
Cervix
Collection
Contamination
ejaculate
Fixation
Penis
Semen
sperm
Studs
swine
swine
aerobic mesophiles
sperm
bacterial load
ejaculate
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Summary:Semen collection has an essential role in the initial bacterial load in boar ejaculates and extended semen. The study aimed to explore the efficacy of an adjusted penis fixation in a semi‐automatic collection system on reducing bacterial contamination of ejaculates in two‐boar studs with different scenarios. Historically, stud A had low levels of bacterial load in raw semen, while stud B had a high level of contamination. A total of 56 mature boars had their semen collected using two methods of penis fixation: (a) Traditional: The penis was fixed directly with the artificial cervix and transferred to the adjustable clamp; (b) Adjusted: The fixation was performed with one gloved‐hand, and after exteriorization, the penis was gripped using the artificial cervix with the other gloved‐hand and transferred to the adjustable clamp. The bacterial load (p = .0045) and the occurrence of ejaculates >231 CFU/ml (p = .0101) were reduced in the Adjusted compared to the Traditional method. Bacterial load was reduced when using the Adjusted method in stud B (p = .0011), which showed a greater occurrence of critical factors for bacterial contamination (p ≤ .0034). The Adjusted method reduced the occurrence of ejaculates >231 CFU/ml when the preputial ostium was dirty (p = .016) and the duration of semen collection was >7 min (p = .022) compared to the Traditional method. In conclusion, the Adjusted penis fixation was efficient in reducing bacterial load of ejaculates, mainly in boar stud B, which had high contamination challenges.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13932