Search Results - "Fernando, M Rohan"

New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes by Fernando, M Rohan, Jiang, Chao, Krzyzanowski, Gary D, Ryan, Wayne L

“…Cell-free DNA (cfDNA) in blood is used as a source of genetic material for noninvasive prenatal and cancer diagnostic assays in clinical practice. Recently we…”
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Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes by Chandel, Dinesh S, Tom, Wesley A, Jiang, Chao, Krzyzanowski, Gary, Fernando, Nirmalee, Olou, Appolinaire, Fernando, M Rohan

“…Circulating miRNA has recently emerged as important biomolecules with potential clinical values as diagnostic markers for several diseases. However, to be used…”
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Genotype Characterization and MiRNA Expression Profiling in Usher Syndrome Cell Lines by Tom, Wesley A, Chandel, Dinesh S, Jiang, Chao, Krzyzanowski, Gary, Fernando, Nirmalee, Olou, Appolinaire, Fernando, M Rohan

“…Usher syndrome (USH) is an inherited disorder characterized by sensorineural hearing loss (SNHL), retinitis pigmentosa (RP)-related vision loss, and vestibular…”
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A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time by Fernando, M Rohan, Jiang, Chao, Krzyzanowski, Gary D, Somer-Shely, Tifany, Ryan, Wayne L

“…This study was undertaken to evaluate a novel method for stabilizing and preserving the original proportion of cell-free fetal DNA (cffDNA) in maternal blood…”
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A New Blood Collection Device Minimizes Cellular DNA Release During Sample Storage and Shipping When Compared to a Standard Device by Norton, Sheila E., Luna, Kristin K., Lechner, Joel M., Qin, Jianbing, Fernando, M. Rohan

“…Background Cell‐free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for…”
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Effects of a novel cell stabilizing reagent on DNA amplification by PCR as compared to traditional stabilizing reagents by Das, Kausik, Fernando, M. Rohan, Basiaga, Sara, Wigginton, Stephanie M., Williams, Tom

“…Stabilization of nucleated blood cells by cell stabilizing reagent (BCT reagent) present in the Cell-Free DNA BCT™ blood collection device and consequent…”
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Mitochondrial thioltransferase (glutaredoxin 2) has GSH-dependent and thioredoxin reductase-dependent peroxidase activities in vitro and in lens epithelial cells by Fernando, M. Rohan, Lechner, Joel M, Löfgren, Stefan, Gladyshev, Vadim N, Lou, Marjorie F

“…Thioltransferase (or Grx) belongs to the oxidoreductase family and is known to regulate redox homeostasis in cells. Mitochondrial Grx2 is a recent discovery,…”
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Effect of Thioltransferase (Glutaredoxin) Deletion on Cellular Sensitivity to Oxidative Stress and Cell Proliferation in Lens Epithelial Cells of Thioltransferase Knockout Mouse by Lofgren, Stefan, Fernando, M. Rohan, Xing, Kui-Yi, Wang, Yin, Kuszynski, Charles A, Ho, Ye-Shih, Lou, Marjorie F

“…To examine the physiological function of the thioltransferase (TTase)/glutathione (GSH) system in the lens using TTase knockout mouse (TTase(-/-)) lens…”
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Analysis of human blood plasma cell-free DNA fragment size distribution using EvaGreen chemistry based droplet digital PCR assays by Fernando, M. Rohan, Jiang, Chao, Krzyzanowski, Gary D., Ryan, Wayne L.

“…Plasma cell-free DNA (cfDNA) fragment size distribution provides important information required for diagnostic assay development. We have developed and…”
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Low molecular weight protein tyrosine phosphatase (LMW-PTP) and its possible physiological functions of redox signaling in the eye lens by Xing, Kuiyi, Raza, Ashraf, Löfgren, Stefan, Fernando, M. Rohan, Ho, Ye-Shih, Lou, Marjorie F.

“…Low molecular weight protein tyrosine phosphatase (LMW-PTP) was cloned from human lens epithelial B3 cells (HLE B3) and the recombinant enzyme was purified to…”
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Regulation of the bioavailability of thioredoxin in the lens by a specific thioredoxin-binding protein (TBP-2) by Liyanage, Namal P.M., Fernando, M. Rohan, Lou, Marjorie F.

“…Thioredoxin (TRx) is known to control redox homeostasis in cells. In recent years, a specific TRx binding protein called thioredoxin binding protein-2 (TBP-2)…”
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P022: Exploring preanalytical factors impacting plasma cell-free miRNA-451 levels by Chandel, Dinesh, Tom, Wesley, Krzyzanowski, Gary, Fernando, M. Rohan

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A novel blood collection device stabilizes cell-free RNA in blood during sample shipping and storage by Qin, Jianbing, Williams, Thomas L, Fernando, M Rohan

“…Cell-free RNA (cfRNA) naturally occurs in blood and has clinical significance. Accurate quantification of these extracellular RNAs in whole blood is hindered…”
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Stabilization of circulating tumor cells in blood using a collection device with a preservative reagent by Qin, Jianbing, Alt, Jodi R, Hunsley, Bradford A, Williams, Thomas L, Fernando, M Rohan

“…The enumeration and characterization of circulating tumor cells (CTCs) in the blood of cancer patients is useful for cancer prognostic and treatment monitoring…”
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Stabilization of cell-free RNA in blood samples using a new collection device by Fernando, M. Rohan, Norton, Sheila E., Luna, Kristin K., Lechner, Joel M., Qin, Jianbing

“…To investigate whether a new blood collection device stabilizes cell-free RNA (cfRNA) in blood post-phlebotomy when compared to collection using K3EDTA tubes…”
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Thioltranferase Mediated Ascorbate Recycling in Human Lens Epithelial Cells by Fernando, M. Rohan, Satake, Makoto, Monnier, Vincent M, Lou, Marjorie F

“…This study was undertaken to investigate whether thioltransferase (TTase) exhibits dehydroascorbate (DHA) reductase activity in human lens epithelial cells…”
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Inactivation and viral load quantitation of human immunodeficiency virus in blood collected into Cyto-Chex® BCT blood collection device by Kwon, Eunhee, Minhas, Veenu, Phiri, Tambudzai, Wood, Charles, Swindells, Susan, Hunsley, Bradford A., Fernando, M. Rohan

“…•We investigated the effect of Cyto-Chex® BCT reagent on HIV inactivation and stability in blood at room temperature.•Cyto-Chex® BCT inactivated HIV in blood…”
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Stabilization of Cellular RNA in Blood During Storage at Room Temperature: A Comparison of Cell-Free RNA BCT® with K3EDTA Tubes by Das, Kausik, Norton, Sheila E., Alt, Jodi R., Krzyzanowski, Gary D., Williams, Thomas L., Fernando, M. Rohan

“…Background Messenger RNA (mRNA) expression levels in blood cells are important in disease diagnosis, prognosis and biomarker discovery research. Accurate…”
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WITHDRAWN: Compatibility of a blood collection tube that stabilizes cell-free DNA with a rapid fluorescence assay by Fernando, Dinith S, Lechner, Joel M, Norton, Sheila E, Qin, Jianbing, Fernando, M Rohan

“…This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier…”
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Induction of Thioltransferase and Thioredoxin/Thioredoxin Reductase Systems in Cultured Porcine Lenses under Oxidative Stress by Moon, Sungchur, Fernando, M. Rohan, Lou, Marjorie F

“…In view of the important antioxidant roles of thioltransferase (TTase), thioredoxin (Trx), and thioredoxin reductase (TR) in the lens, the present study was…”
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