Search Results - "Fernando, Lawrence P."

Nanoscale 3D Tracking with Conjugated Polymer Nanoparticles by Yu, Jiangbo, Wu, Changfeng, Sahu, Sushant P, Fernando, Lawrence P, Szymanski, Craig, McNeill, Jason

“…Small (∼15 nm diameter), highly fluorescent conjugated polymer nanoparticles were evaluated for nanoscale 2D and 3D tracking applications. Nanoparticles…”
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Mechanism of Cellular Uptake of Highly Fluorescent Conjugated Polymer Nanoparticles by Fernando, Lawrence P, Kandel, Prakash K, Yu, Jiangbo, McNeill, Jason, Ackroyd, P. Christine, Christensen, Kenneth A

“…Conjugated polymer nanoparticles are formed by precipitation of highly fluorescent conjugated polymers to form small nanoparticles with extremely bright…”
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Surface-Enhanced Raman Scattering Detection of pH with Silica-Encapsulated 4‑Mercaptobenzoic Acid-Functionalized Silver Nanoparticles by Wang, Fenglin, Widejko, Ryan G, Yang, Zhiqiang, Nguyen, KhanhVan T, Chen, Hongyu, Fernando, Lawrence P, Christensen, Kenneth A, Anker, Jeffrey N

“…Sensors based upon surface-enhanced Raman spectroscopy (SERS) are attractive because they have narrow, vibrationally specific spectral peaks that can be…”
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Incorporating functionalized polyethylene glycol lipids into reprecipitated conjugated polymer nanoparticles for bioconjugation and targeted labeling of cells by Kandel, Prakash K, Fernando, Lawrence P, Ackroyd, P Christine, Christensen, Kenneth A

“…We report a simple and rapid method to prepare extremely bright, functionalized, stable, and biocompatible conjugated polymer nanoparticles incorporating…”
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Formulation and characterization of poly(propylacrylic acid)/poly(lactic‐co‐glycolic acid) blend microparticles for pH‐dependent membrane disruption and cytosolic delivery by Fernando, Lawrence P., Lewis, Jamal S., Evans, Brian C., Duvall, Craig L., Keselowsky, Benjamin G.

“…Poly(lactic‐co‐glycolic acid) (PLGA) is widely used as a vehicle for delivery of pharmaceutically relevant payloads. PLGA is readily fabricated as a nano‐ or…”
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High molecular weight kininogen and factor XII binding to endothelial cells and astrocytes by Fernando, Lawrence P, Natesan, Snehlatha, Joseph, Kusumam, Kaplan, Allen P

“…We have quantitated the binding of high molecular weight kininogen (HK) to human microvascular endothelial cells of lung and dermal origin as well as to…”
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Dynamic transcriptional response of Escherichia coli to inclusion body formation by Baig, Faraz, Fernando, Lawrence P., Salazar, Mary Alice, Powell, Rhonda R., Bruce, Terri F., Harcum, Sarah W.

“…ABSTRACT Escherichia coli is used intensively for recombinant protein production, but one key challenge with recombinant E. coli is the tendency of recombinant…”
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The relative brightness of PEG lipid-conjugated polymer nanoparticles as fluid-phase markers in live cells by Fernando, Lawrence P., Kandel, Prakash K., Ackroyd, P. Christine, Christensen, Kenneth A.

“…While conjugated polymer nanoparticles (CPNs) have been widely touted as ultra-bright labels for biological imaging, no direct comparative measurements of…”
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The effects of protein solubility on the RNA Integrity Number (RIN) for recombinant Escherichia coli by Salazar, Mary Alice, Fernando, Lawrence P., Baig, Faraz, Harcum, Sarah W.

“…•Recombinant E. coli gene expression analysis requires high quality RNA.•RNA Integrity Number (RIN) evaluates RNA quality for gene expression analysis.•Soluble…”
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Poly(2‐propylacrylic acid)/poly(lactic‐co‐glycolic acid) blend microparticles as a targeted antigen delivery system to direct either CD4+ or CD8+ T cell activation by Yang, Lirong, Bracho‐Sanchez, Evelyn, Fernando, Lawrence P., Lewis, Jamal S., Carstens, Matthew R., Duvall, Craig L., Keselowsky, Benjamin G.

“…Poly(lactic‐co‐glycolic acid) (PLGA) based microparticles (MPs) are widely investigated for their ability to load a range of molecules with high efficiency,…”
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Assembly, activation, and signaling by kinin-forming proteins on human vascular smooth muscle cells by Fernando, Anthony N, Fernando, Lawrence P, Fukuda, Yu, Kaplan, Allen P

“…Cardiovascular disease is the number one cause of death in the United States. Vascular smooth muscle cells (VSMC) are an important constituent of the vessel…”
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Quantitation of Gene-Specific DNA Damage by Competitive PCR by Fernando, Lawrence P., Kurian, Philip J., Fidan, Melihat, Fernandes, Daniel J.

“…A sensitive assay for quantitating DNA damage within individual genes would be a valuable tool for identifying the molecular mechanisms of disease and the…”
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Avian metallothioneins: structure, regulation and evolution by Andrews, G K, Fernando, L P, Moore, K L, Dalton, T P, Sobieski, R J

“…This article reviews studies of the molecular biology of the avian metallothionein (MT) genes. Analysis of cloned genes and/or cDNAs from chicken, turkey,…”
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Increased prostacyclin and PGE2 stimulated cAMP production by macrophages from endotoxin-tolerant rats by Makhlouf, M A, Fernando, L P, Gettys, T W, Halushka, P V, Cook, J A

“…Sublethal administration of lipopolysaccharide (LPS) renders rats tolerant to multiple lethal stimuli. Tolerant macrophages exhibit differential alterations in…”
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Activation of the Chicken Metallothionein Promoter by Metals and Oxidative Stress in Cultured Cells and Transgenic Mice by Dalton, Tim, Paria, Bibhash C., Fernando, Lawrence P., Huet-Hudson, Yvette M., Dey, Sudhansu K., Andrews, Glen K.

“…Cis-acting elements in the chicken metallothionein promoter were tested for their ability to direct responses of reporter genes to metal ions and oxidative…”
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Protective effect of tyrphostin AG-556 on shock induced by endotoxin or gram positive bacteria by Ogura, S, Umegaki, O, Genovese, F, Fernando, L P, Cochran, J B, Romeo, C M, Cook, J A

“…The effects of tyrphostin AG-556 (TYR), a tyrosine kinase inhibitor, were evaluated on shock induced by lipopolysaccharide (LPS) or group B streptococcus (GBS)…”
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Tolerance to LPS decreases macrophage G protein content by Fernando, L P, Makhlouf, M, Fernando, A N, Ashton, S, Halushka, P V, Cook, J A

“…The effects of tolerizing doses of LPS on mRNA and protein levels of three different G protein subunits were investigated to understand the mechanism(s)…”
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Cloning and expression of an avian metallothionein-encoding gene by Fernando, L P, Andrews, G K

“…The chicken metallothionein gene (cMT), isolated from a chicken genomic DNA phage lambda library, was found to be approximately 1.5 kb in length and to consist…”
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Rapid Site-Directed Mutagenesis by a Method That Selects for Full Length Mutated DNA by Wang, Cheng C., Fernando, Lawrence P., Low, Philip S.

“…Site-directed mutagenesis is a powerful tool for exploring structure-function relationships of protein and nucleic acids. Traditional methods of site-specific…”
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