Search Results - "Fernandez–Lafuente, Roberto"

Biotechnological Applications of Proteases in Food Technology by Tavano, Olga Luisa, Berenguer‐Murcia, Angel, Secundo, Francesco, Fernandez‐Lafuente, Roberto

“…This review presents some of the hottest topics in biotechnological applications: proteases in biocatalysis. Obviously, one of the most relevant areas of…”
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Immobilization on octyl‐agarose beads and some catalytic features of commercial preparations of lipase a from Candida antarctica (Novocor ADL): Comparison with immobilized lipase B from Candida antarctica by Arana‐Peña, Sara, Lokha, Yuliya, Fernández‐Lafuente, Roberto

“…Lipase A from Candida antarctica (CALA, commercialized as Novocor ADL) was immobilized on octyl‐agarose, which is a very useful support for lipase…”
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Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance by Garcia-Galan, Cristina, Berenguer-Murcia, Ángel, Fernandez-Lafuente, Roberto, Rodrigues, Rafael C.

“…Enzyme biocatalysis plays a very relevant role in the development of many chemical industries, e.g., energy, food or fine chemistry. To achieve this goal,…”
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Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes by Rodrigues, Rafael C., Berenguer-Murcia, Ángel, Fernandez-Lafuente, Roberto

“…Chemical modification and immobilization of enzymes have been usually considered unrelated tools to improve biocatalyst features. However, there are many…”
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The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support by Abellanas‐Perez, Pedro, Carballares, Diego, Rocha‐Martin, Javier, Fernandez‐Lafuente, Roberto

“…In this article, we have analyzed the interactions between enzyme crowding on a given support and its chemical modification (ethylenediamine modification via…”
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Kinetic resolution of drug intermediates catalyzed by lipase B from Candida antarctica immobilized on immobead‐350 by Pinheiro, Maísa Pessoa, Rios, Nathalia Saraiva, Fonseca, Thiago de S., Bezerra, Francisco de Aquino, Rodríguez‐Castellón, Enrique, Fernandez‐Lafuente, Roberto, Carlos de Mattos, Marcos, dos Santos, José C. S., Gonçalves, Luciana R. B.

“…Novozyme 435, which is a commercial immobilized lipase B from Candida antarctica (CALB), has been proven to be inadequate for the kinetic resolution of…”
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Structure and activity of magnetic cross-linked enzyme aggregates of bovine carbonic anhydrase as promoters of enzymatic CO2 capture by Peirce, Sara, Russo, Maria Elena, Isticato, Rachele, Lafuente, Roberto Fernández, Salatino, Piero, Marzocchella, Antonio

“…[Display omitted] •Bovine carbonic anhydrase CLEA were prepared with 84% enzyme immobilization yield.•Optimal conditions for enzyme precipitation and…”
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Rapid and high yield production of phospholipids enriched in CLA via acidolysis: The critical role of the enzyme immobilization protocol by Verdasco-Martín, Carlos M., Corchado-Lopo, Carlos, Fernández-Lafuente, Roberto, Otero, Cristina

“…•Advanced selective synthesis of CLA rich phosphatidylcholines.•Selective acydolysis of phospholipids with new immobilized Lecitase Ultra.•Rapid, selective and…”
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Cooperativity of covalent attachment and ion exchange on alcalase immobilization using glutaraldehyde chemistry: Enzyme stabilization and improved proteolytic activity by Ait Braham, Sabrina, Hussain, Fouzia, Morellon‐Sterling, Roberto, Kamal, Shagufta, Kornecki, Jakub F., Barbosa, Oveimar, Kati, Djamel Edine, Fernandez‐Lafuente, Roberto

“…Alcalase was scarcely immobilized on monoaminoethyl‐N‐aminoethyl (MANAE)‐agarose beads at different pH values (<20% at pH 7). The enzyme did not immobilize on…”
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Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features by Sara Arana-Peña, Yuliya Lokha, Roberto Fernández-Lafuente

“…Eversa is an enzyme recently launched by Novozymes to be used in a free form as biocatalyst in biodiesel production. This paper shows for first time the…”
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Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug‐flow packed‐bed continuous reactor by Poppe, Jakeline Kathiele, Matte, Carla Roberta, de Freitas, Vitória Olave, Fernandez‐Lafuente, Roberto, Rodrigues, Rafael C., Záchia Ayub, Marco Antônio

“…This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed‐bed continuous reactor, using mixtures of immobilized lipases…”
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Prolongation of secondary drying step of phospholipid lyophilization greatly improves acidolysis reactions catalyzed by immobilized lecitase ultra by Verdasco-Martín, Carlos M., Corchado-Lopo, Carlos, Fernández-Lafuente, Roberto, Otero, Cristina

“…•Improbed performance of Acidolisis of phosphatidyl choline.•Enhanced method of synthesis of CLA rich phospholids.•Acidolysis of phosphatidyl choline with…”
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Stability/activity features of the main enzyme components of rohapect 10L by Dal Magro, Lucas, Kornecki, Jakub F., Klein, Manuela P., Rodrigues, Rafael C., Fernandez‐Lafuente, Roberto

“…Rohapect 10L is an enzyme cocktail commercialized for juice clarification. Here, we characterized the activity and stability of five enzymatic activities…”
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1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates by Ramos, Margarita D., Miranda, Letícia P., Giordano, Raquel L. C., Fernandez‐Lafuente, Roberto, Kopp, William, Tardioli, Paulo W.

“…The preparation of crosslinked aggregates of pancreatic porcine lipase (PPL‐CLEA) was systematically studied, evaluating the influence of three precipitants…”
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Intraparticle Macromolecular Migration Alters the Structure and Function of Proteins Reversibly Immobilized on Porous Microbeads by Diamanti, Eleftheria, Arana‐Peña, Sara, Ramos‐Cabrer, Pedro, Comino, Natalia, Carballares, Diego, Fernandez‐Lafuente, Roberto, López‐Gallego, Fernando

“…While migration of reversibly immobilized proteins across the volume of supports is investigated in conditions where an external force is applied or under…”
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Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids by Cipolatti, Eliane Pereira, Pinto, Martina Costa Cerqueira, Robert, Julia de Macedo, da Silva, Tabita Pizoni, Beralto, Thamires da Costa, Santos, Jorge G. F., de Castro, Rui de Paula Vieira, Fernandez‐Lafuente, Roberto, Manoel, Evelin Andrade, Pinto, José Carlos, Freire, Denise Maria Guimarães

“…ABSTRACT A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as…”
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Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalyst by Fernandez-Lafuente, Roberto

“…The lipase from Thermomyces laguginosus (formerly Humicola laguginosa) (TLL) is a basophilic and noticeably thermostable enzyme, commercially available in both…”
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Preparation of immobilized/stabilized biocatalysts of β‐glucosidases from different sources: Importance of the support active groups and the immobilization protocol by Andrades, Diandra, Graebin, Natália G., Ayub, Marco A. Z., Fernandez‐Lafuente, Roberto, Rodrigues, Rafael C.

“…β‐Glucosidases from two different commercial preparations, Pectinex Ultra SP‐L and Celluclast® 1.5L, were immobilized on divinylsulfone (DVS) supports at pH…”
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Parameters necessary to define an immobilized enzyme preparation by Boudrant, Joseph, Woodley, John M., Fernandez-Lafuente, Roberto

“…[Display omitted] •Understanding of the enzyme immobilization is required to optimize the processes.•Immobilization parameters and courses become critical to…”
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Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance by Hernandez, Karel, Fernandez-Lafuente, Roberto

“…Mutagenesis and immobilization are usually considered to be unrelated techniques with potential applications to improve protein properties. However, there are…”
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