Neutrophils from patients with the cardiac clinical form of Chagas disease release less NETs than neutrophils from healthy individuals

Neutrophils from patients with the cardiac clinical form of Chagas disease release less NETs than neutrophils from healthy individuals

Introduction Chagas disease (CD) is a global health concern with approximately 12 000 deaths per year worldwide. In the chronic phase, about 30% of patients develop the cardiac clinical form, which presents symptoms associated with the presence of inflammatory cells in the cardiac tissue. Neutrophil...

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Published in:Parasite immunology Vol. 43; no. 4; pp. e12821 - n/a
Main Authors: Duarte de Almeida, Valéria, Câmara, Eduardo B. L., Castro, Marcelo Augusto A., Pimenta, Suyane Maria P., Hernandes, Bruna Luiza T., Paiva, Dayane Carla C., Andrade, Cléber M., Nascimento, Ellany G. C., Fernandes, Thales Allyrio A. M., Andrade, Micássio F.
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-04-2021
Subjects:
Chagas disease
Death
Degranulation
Deoxyribonucleic acid
DNA
Elastase
Heart diseases
Immune response
Inflammation
Leukocytes (neutrophilic)
NETs
neutrophil
Neutrophils
Public health
Trypanosoma cruzi
elastase
Chagas disease
NETs
immune response
neutrophil
Trypanosoma cruzi
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Summary:Introduction Chagas disease (CD) is a global health concern with approximately 12 000 deaths per year worldwide. In the chronic phase, about 30% of patients develop the cardiac clinical form, which presents symptoms associated with the presence of inflammatory cells in the cardiac tissue. Neutrophils are inflammatory cells able to modulate the chronic immune response against pathogens. These cells are capable of interacting with Trypanosoma cruzi, the aetiological agent of CD, and perform several effector functions, such as NET release. However, few studies have been carried out to investigate the role of these cells in the disease. Aims To investigate the release of NETs by neutrophils from CD patients by measuring the amount of DNA and elastase released. Methods and results Measurement of DNA release by neutrophils from chronic CD patients presenting the indeterminate (IND group; n = 18) and cardiac (CARD group; n = 15) clinical forms and nonchagasic subjects (n = 18) stimulated with soluble antigen of T. cruzi was quantified using the Quant‐iT™ PicoGreen® dsDNA assay kit. Patients from CARD group release less DNA (117.3 ± 21.85 ng/mL; *P = .0131) than neutrophils from control (177.7 ± 58.41 ng/mL). Elastase enzyme degranulation was measured using the substrate N‐methoxysuccinyl‐Ala‐Ala‐Pro‐Val p‐nitroanilide (SAAVNA). Absorbance values of elastase degranulation activity showed that only cells from healthy individuals presented a high release profile of elastase. Also, we found a negative correlation between DNA released concentration and risk of death (r = −.6574; *P = .0173); the lower the neutrophil DNA release from chagasic patients with cardiac event, the higher the risk of death. Conclusion These preliminary data show that patients with the cardiac form of CD release less NETs than nonchagasic individuals, raising the possibility that lower release of NETs enhances risk of death in CD patients with cardiac events.
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ISSN:0141-9838
1365-3024
DOI:10.1111/pim.12821