Reversal of diet-induced insulin resistance with a single bout of exercise in the rat: the role of PTP1B and IRS-1 serine phosphorylation

Lifestyle interventions including exercise programmes are cornerstones in the prevention of obesity-related diabetes. In this study, we demonstrate that a single bout of exercise inhibits high-fat diet-induced insulin resistance. Diet-induced obesity (DIO) increased the expression and activity of th...

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Published in:	The Journal of physiology Vol. 577; no. 3; pp. 997 - 1007
Main Authors:	Ropelle, Eduardo R., Pauli, José R., Prada, Patrícia O., De Souza, Cláudio T., Picardi, Paty K., Faria, Marcel C., Cintra, Dennys E., Fernandes, Maria Fernanda de A., Flores, Marcelo B., Velloso, Lício A., Saad, Mario J. A., Carvalheira, José B. C.
Format:	Journal Article
Language:	English
Published:	Oxford, UK The Physiological Society 15-12-2006 Blackwell Publishing Ltd Blackwell Science Inc
Subjects:	Animals Dietary Fats - administration & dosage Dose-Response Relationship, Drug I-kappa B Proteins - antagonists & inhibitors Insulin - metabolism Insulin Receptor Substrate Proteins Insulin Resistance JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors JNK Mitogen-Activated Protein Kinases - metabolism Male Muscle, Skeletal - metabolism Obesity - etiology Obesity - metabolism Obesity - physiopathology Phosphoproteins - chemistry Phosphoproteins - metabolism Phosphorylation Physical Conditioning, Animal Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatases - metabolism Rats Rats, Wistar Recovery of Function Serine - metabolism Signal Transduction Skeletal Muscle and Exercise Swimming
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Summary:	Lifestyle interventions including exercise programmes are cornerstones in the prevention of obesity-related diabetes. In this study, we demonstrate that a single bout of exercise inhibits high-fat diet-induced insulin resistance. Diet-induced obesity (DIO) increased the expression and activity of the protein tyrosine phosphatase 1B (PTP1B) and attenuated insulin signalling in gastrocnemius muscle of rats, a phenomenon which was reversed by a single session of exercise. In addition, DIO was observed to lead to serine phosphorylation of insulin receptor substrate 1 (IRS-1), which was also reversed by exercise in muscle in parallel with a reduction in c-Jun N-terminal kinase (JNK) activity. Thus, acute exercise increased the insulin sensitivity during high-fat feeding in obese rats. Overall, these results provide new insights into the mechanism by which exercise restores insulin sensitivity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3751 1469-7793
DOI:	10.1113/jphysiol.2006.120006