In situ detection of SOCS and cytokine expression in the uterine cervix from HIV/HPV coinfected women

The purpose of this study was to look for associations between a newly described class of suppressors of cytokine signaling (SSI/SOCS) and cytokine expression in the uterine cervix from HIV/HPV coinfected women. We examined the pro-inflammatory cytokines TNF-α and IL-6 since their expressions are li...

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Published in:	Experimental and molecular pathology Vol. 81; no. 1; pp. 42 - 47
Main Authors:	Nicol, A.F., Nuovo, G.J., Wang, Y., Grinsztejn, B., Tristão, A., Russomano, F., Perez, M.A., Lapa e Silva, J.R., Fernandes, A.T.G., Gage, J.R., Martínez-Maza, O., Bonecini-Almeida, M.G.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-08-2006 Elsevier
Subjects:	Adult B7-2 Antigen - genetics B7-2 Antigen - metabolism Biological and medical sciences Cervix Uteri - immunology Cervix Uteri - metabolism Cervix Uteri - pathology Cytokines - genetics Cytokines - metabolism DNA, Viral - analysis Female HIV Infections - complications HIV Infections - immunology HIV Infections - metabolism HIV-1 - isolation & purification HIV-1/HPV coinfection Human viral diseases Humans In situ RT-PCR Infectious diseases Interleukin-6 - genetics Interleukin-6 - metabolism Investigative techniques, diagnostic techniques (general aspects) Medical sciences Papillomaviridae - isolation & purification Papillomavirus Infections - complications Papillomavirus Infections - immunology Papillomavirus Infections - metabolism Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - analysis RNA, Messenger - metabolism SSI/SOCS Suppressor of Cytokine Signaling Proteins - genetics Suppressor of Cytokine Signaling Proteins - metabolism Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Uterine cervix Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids In situ RT-PCR Uterine cervix SSI/SOCS HIV-1/HPV coinfection Mixed infection RNA-directed DNA polymerase HIV-1 virus In situ Papovaviridae Papillomavirus Human papillomavirus Adult Female Diagnosis Woman Reverse transcription polymerase chain reaction Human Immunopathology Enzyme Transferases Cytokine Retroviridae AIDS Immune deficiency Lentivirus Infection Virus Nucleotidyltransferases Anatomic pathology Viral disease Suppressor cytokine signaling Human immunodeficiency virus Detection
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Summary:	The purpose of this study was to look for associations between a newly described class of suppressors of cytokine signaling (SSI/SOCS) and cytokine expression in the uterine cervix from HIV/HPV coinfected women. We examined the pro-inflammatory cytokines TNF-α and IL-6 since their expressions are linked and responsible for many aspects of both localized and systemic inflammatory responses. Further, expression of SSI/SOCS has been implicated in the negative feedback regulation of cytokine receptor signaling. PCR-amplified HIV-1 cDNA was noted mainly in the stroma, showing a perivascular distribution, and most of the infected cells colabeled with the macrophage marker CD68. The distribution of IL-6 and TNF-α was in the same area to HIV-1 and much greater than normal cervices from women with no evidence of viral infection. SOCS/SSI-1 and -3 mRNA positive cells in the uterine cervix were commonly detected in these noninfected cervical tissues; however, very few cells that contained SOCS were evident in areas where HIV-1, TNF-α, and IL-6 expressing cells were found. This suggests that viral-related suppression of SOCS/SSI-1-3 expression may be a factor in the marked local enhancement of TNF-α and IL-6 production which, in turn, may help facilitate viral spread; however, further studies should be done in order to elucidate the exact mechanisms of SOCS in the cervix.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4800 1096-0945
DOI:	10.1016/j.yexmp.2006.01.002