Search Results - "Fernández Lorente, Gloria"

Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements by Carrasco-López, César, Godoy, César, de las Rivas, Blanca, Fernández-Lorente, Gloria, Palomo, José M., Guisán, José M., Fernández-Lafuente, Roberto, Martínez-Ripoll, Martín, Hermoso, Juan A.

“…The bacterial thermoalkalophilic lipases that hydrolyze saturated fatty acids at 60–75 °C and pH 8–10 are grouped as the lipase family I.5. We report here the…”
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Improvement of Enzyme Properties with a Two-Step Immobilizaton Process on Novel Heterofunctional Supports by Mateo, Cesar, Bolivar, Juan Manuel, Godoy, Cesar A, Rocha-Martin, Javier, Pessela, Benevides C, Curiel, Jose Antonio, Muñoz, Rosario, Guisan, Jose M, Fernández-Lorente, Gloria

“…Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contain a high concentration of groups (such as quaternary ammonium groups,…”
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Hydrolysis of Tannic Acid Catalyzed by Immobilized−Stabilized Derivatives of Tannase from Lactobacillus plantarum by Curiel, Jose Antonio, Betancor, Lorena, de las Rivas, Blanca, Muñoz, Rosario, Guisan, Jose M, Fernández-Lorente, Gloria

“…A recombinant tannase from Lactobacillus plantarum, overexpressed in Escherichia coli, was purified in a single step by metal chelate affinity chromatography…”
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Purification of different lipases from Aspergillus niger by using a highly selective adsorption on hydrophobic supports by Fernandez-Lorente, G, Ortiz, C, Segura, R.L, Fernandez-Lafuente, R, Guisan, J.M, Palomo, J.M

“…In this manuscript, we have purified three different lipases from crude preparations from Aspergillus niger in a simple fashion, secluding the esterases and…”
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Separation and Immobilization of Lipase from Penicillium simplicissimum by Selective Adsorption on Hydrophobic Supports by Cunha, Aline G, Fernández-Lorente, Gloria, Gutarra, Melissa L. E, Bevilaqua, Juliana V, Almeida, Rodrigo V, Paiva, Lúcia M. C, Fernández-Lafuente, Roberto, Guisán, Jose M, Freire, Denise M. G

“…Lipases are an enzyme class of a great importance as biocatalysts applied to organic chemistry. However, it is still necessary to search for new enzymes with…”
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Various Strategies for the Immobilization of a Phospholipase C from Bacillus cereus for the Modulation of Its Biochemical Properties by Abdelkader, Ines, Guisán, Jose M, Sayari, Adel, Fernández-Lorente, Gloria

“…In this study, the effect of various immobilization methods on the biochemical properties of phospholipase C (PLC) from obtained from the oily soil located in…”
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Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins by Mateo, César, Palomo, José M., Fuentes, Manuel, Betancor, Lorena, Grazu, Valeria, López-Gallego, Fernando, Pessela, Benevides C.C., Hidalgo, Aurélio, Fernández-Lorente, Gloria, Fernández-Lafuente, Roberto, Guisán, José M.

“…Glyoxyl agarose is constituted by quite thick agarose fibres containing a large number of very stable aldehyde groups attached to the support by very short…”
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Immobilization of Moniliella spathulata R25L270 Lipase on Ionic, Hydrophobic and Covalent Supports: Functional Properties and Hydrolysis of Sardine Oil by Souza, Lívia T de A, Moreno-Perez, Sonia, Fernández Lorente, Gloria, Cipolatti, Eliane P, de Oliveira, Débora, Resende, Rodrigo R, Pessela, Benevides C

“…The oleaginous yeast R25L270 was the first yeast able to grow and produce extracellular lipase using Macaúba ( ) cake as substrate. The novel lipase was…”
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A novel halophilic lipase, LipBL, showing high efficiency in the production of eicosapentaenoic acid (EPA) by Pérez, Dolores, Martín, Sara, Fernández-Lorente, Gloria, Filice, Marco, Guisán, José Manuel, Ventosa, Antonio, García, María Teresa, Mellado, Encarnación

“…Among extremophiles, halophiles are defined as microorganisms adapted to live and thrive in diverse extreme saline environments. These extremophilic…”
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Biocatalysts Based on Immobilized Lipases for the Production of Ethyl Esters of Fatty Acids including Bioactive Gamma-Linolenic Acid from Borage Oil by Gonzalo Berzal, Martín García-González, Natalia Castejón, Paz García-García, Gloria Fernández-Lorente, Francisco J. Señoráns

“…In the present work, borage oil (Borago officinalis) was used as the main source of gamma linolenic acid (GLA) to obtain ethyl esters by enzymatic ethanolysis…”
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Release of Omega-3 Fatty Acids by the Hydrolysis of Fish Oil Catalyzed by Lipases Immobilized on Hydrophobic Supports by Fernández-Lorente, Gloria, Betancor, Lorena, Carrascosa, Alfonso V., Guisán, Jose M.

“…The release of omega-3 fatty acids by the mild enzymatic hydrolysis of sardine oil was studied. The derivatives of different lipases physically adsorbed on…”
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Stabilization of Lecitase Ultra® by Immobilization and Fixation of Bimolecular Aggregates. Release of Omega-3 Fatty Acids by Enzymatic Hydrolysis of Krill Oil by Daniel Andrés-Sanz, Cristina Fresan, Gloria Fernández-Lorente, Javier Rocha-Martín, Jose M. Guisán

“…Lecitase Ultra® solutions are mainly composed of bimolecular aggregates of two open structures of the enzyme. The immobilization and fixation of these…”
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Modulation of the Selectivity of Immobilized Lipases by Chemical and Physical Modifications: Release of Omega-3 Fatty Acids from Fish Oil by Fernández-Lorente, Gloria, Betancor, Lorena, Carrascosa, Alfonso V, Palomo, Jose M, Guisan, Jose M

“…Three different lipases (from Candida antarctica fraction B (CALB), Thermomyces lanuginose (TLL), and Rhizomucor miehei (RML)) were immobilized by two…”
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Modulation of Immobilized Lipase Enantioselectivity via Chemical Amination by Palomo, Jose M., Fernández-Lorente, Gloria, Guisán, Jose M., Fernández-Lafuente, Roberto

“…The aspartic and glutamic carboxylic groups of the surface of three different immobilized lipases (those from Candida antarctica (form B) (CAL‐B), Thermomyces…”
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Hydrolysis of Fish Oil by Lipases Immobilized Inside Porous Supports by Fernández-Lorente, Gloria, Pizarro, Carolina, López-Vela, Dolores, Betancor, Lorena, Carrascosa, Alfonso V, Pessela, Benevides, Guisan, Jose M

“…A new assay was designed to measure the release of omega-3 acids [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] from the hydrolysis of sardine…”
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Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment by Mateo, Cesar, Abian, Olga, Fernández-Lorente, Gloria, Pedroche, Justo, Fernández-Lafuente, Roberto, Guisan, Jose M.

“…Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme penicillin G acylase (PGA) via multipoint covalent attachment. These…”
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Epoxy-Amino Groups:  A New Tool for Improved Immobilization of Proteins by the Epoxy Method by Mateo, Cesar, Torres, Rodrigo, Fernández-Lorente, Gloria, Ortiz, Claudia, Fuentes, Manuel, Hidalgo, Aurelio, López-Gallego, Fernando, Abian, Olga, Palomo, Jose M., Betancor, Lorena, Pessela, Benevides C. C., Guisan, José M., Fernández-Lafuente, Roberto

“…The properties of a new commercially available amino-epoxy support (amino-epoxy-Sepabeads) for immobilizing enzymes have been compared to those of conventional…”
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Immobilization of lipases by selective adsorption on hydrophobic supports by Fernandez-Lafuente, Roberto, Armisén, Pilar, Sabuquillo, Pilar, Fernández-Lorente, Gloria, M. Guisán, José

“…The preparation of immobilized derivatives of lipases that may be useful to develop industrial processes of organic synthesis is an exciting field of research…”
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Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties by Fernández-Lorente, Gloria, Palomo, José M., Fuentes, Manuel, Mateo, Cesar, Guisán, José M., Fernández-Lafuente, Roberto

“…It has been found that lipase from Pseudomonas fluorescens (PFL) is able to aggregate into bimolecular structures (MW around 66 kD) even at moderate enzyme…”
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Characterization of a tannase from Emericela nidulans immobilized on ionic and covalent supports for propyl gallate synthesis by Gonçalves, Heloísa Bressan, Jorge, João Atílio, Pessela, Benevides Costa, Lorente, Glória Fernandez, Guisán, José Manuel, Guimarães, Luis Henrique Souza

“…The extracellular tannase from Emericela nidulans was immobilized on different ionic and covalent supports. The derivatives obtained using DEAE-Sepharose and…”
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