Search Results - "Fernández‐Lafuente, Roberto"

Modifying enzyme activity and selectivity by immobilization by Rodrigues, Rafael C, Ortiz, Claudia, Berenguer-Murcia, Ángel, Torres, Rodrigo, Fernández-Lafuente, Roberto

“…Immobilization of enzymes may produce alterations in their observed activity, specificity or selectivity. Although in many cases an impoverishment of the…”
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Comparison of the immobilization of lipase from Pseudomonas fluorescens on divinylsulfone or p-benzoquinone activated support by Rios, Nathalia Saraiva, Neto, Davino M. Andrade, dos Santos, José Cleiton Sousa, Fechine, Pierre Basílio Almeida, Fernández-Lafuente, Roberto, Gonçalves, Luciana Rocha Barros

“…NiZnFe2O4 superparamagnetic nanoparticles were coated with silica by impregnation with tetraethoxysilane (TEOS) and further activated with divinylsulfone (DVS)…”
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Additively Manufactured 3D Micro-bioelectrodes for Enhanced Bioelectrocatalytic Operation by Jodeiri, Keyvan, Foerster, Aleksandra, Trindade, Gustavo F., Im, Jisun, Carballares, Diego, Fernández-Lafuente, Roberto, Pita, Marcos, De Lacey, Antonio L., Parmenter, Christopher D, Tuck, Christopher

“…The drive toward miniaturization of enzyme-based bioelectronics established a need for three-dimensional (3D) microstructured electrodes, which are difficult…”
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Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads by Melo, Ana D Q, Silva, Francisco F M, Dos Santos, José C S, Fernández-Lafuente, Roberto, Lemos, Telma L G, Dias Filho, Francisco A

“…Enzymes serve as biocatalysts for innumerable important reactions, however, their application has limitations, which can in many cases be overcome by using…”
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Different mechanisms of protein immobilization on glutaraldehyde activated supports: Effect of support activation and immobilization conditions by Betancor, Lorena, López-Gallego, Fernando, Hidalgo, Aurelio, Alonso-Morales, Noelia, Mateo, Gisela Dellamora-Ortiz Cesar, Fernández-Lafuente, Roberto, Guisán, Jose M.

“…The precise control of the conditions during support activation with glutaraldehyde has enabled the modification of the amino groups of the matrix with one or…”
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Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements by Carrasco-López, César, Godoy, César, de las Rivas, Blanca, Fernández-Lorente, Gloria, Palomo, José M., Guisán, José M., Fernández-Lafuente, Roberto, Martínez-Ripoll, Martín, Hermoso, Juan A.

“…The bacterial thermoalkalophilic lipases that hydrolyze saturated fatty acids at 60–75 °C and pH 8–10 are grouped as the lipase family I.5. We report here the…”
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Chemoenzymatic Synthesis of the New 3-((2,3-Diacetoxypropanoyl)oxy)propane-1,2-diyl Diacetate Using Immobilized Lipase B from Candida antarctica and Pyridinium Chlorochromate as an Oxidizing Agent by Plata, Esteban, Ruiz, Mónica, Ruiz, Jennifer, Ortiz, Claudia, Castillo, John J, Fernández-Lafuente, Roberto

“…To exploit the hydrolytic activity and high selectivity of immobilized lipase B from on octyl agarose (CALB-OC) in the hydrolysis of triacetin and also to…”
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Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability by Pedroche, Justo, del Mar Yust, Maria, Mateo, Cesar, Fernández-Lafuente, Roberto, Girón-Calle, Julio, Alaiz, Manuel, Vioque, Javier, Guisán, José M., Millán, Francisco

“…The immobilization of trypsin, chymotrypsin and carboxypeptidase A using 4 and 10% glyoxyl-agarose beads at different times of incubation was investigated…”
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Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity Problems by Murilo Amaral-Fonseca, Willian Kopp, Raquel de Lima Camargo Giordano, Roberto Fernández-Lafuente, Paulo Waldir Tardioli

“…The preparation of Cross-Linked Enzyme Aggregates (CLEAs) is a simple and cost-effective technique capable of generating insoluble biocatalysts with high…”
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Synergy of Ion Exchange and Covalent Reaction: Immobilization of Penicillin G Acylase on Heterofunctional Amino-Vinyl Sulfone Agarose by Thays N. da Rocha, Roberto Morellon-Sterling, Luciana R. B. Gonçalves, Juan M. Bolivar, Andrés R. Alcántara, Javier Rocha-Martin, Roberto Fernández-Lafuente

“…Agarose-vinyl sulfone (VS) beads have proven to be a good support to immobilize several enzymes. However, some enzymes are hardly immobilized on it. This is…”
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A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports by Bastida, Agatha, Sabuquillo, Pilar, Armisen, Pilar, Fernández-Lafuente, Roberto, Huguet, Joan, Guisán, José M.

“…A number of bacterial lipases can be immobilized in a rapid and strong fashion on octyl‐agarose gels (e.g., lipases from Candida antarctica, Pseudomonas…”
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Epoxy-Amino Groups:  A New Tool for Improved Immobilization of Proteins by the Epoxy Method by Mateo, Cesar, Torres, Rodrigo, Fernández-Lorente, Gloria, Ortiz, Claudia, Fuentes, Manuel, Hidalgo, Aurelio, López-Gallego, Fernando, Abian, Olga, Palomo, Jose M., Betancor, Lorena, Pessela, Benevides C. C., Guisan, José M., Fernández-Lafuente, Roberto

“…The properties of a new commercially available amino-epoxy support (amino-epoxy-Sepabeads) for immobilizing enzymes have been compared to those of conventional…”
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Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: Evaluation of a novel biocatalyst in organic media by Wilson, Lorena, Illanes, Andrés, Pessela, Benevides C. C., Abian, Olga, Fernández-Lafuente, Roberto, Guisán, José M.

“…The encapsulation of crosslinked enzyme aggregates (CLEA) of penicillin G acylase into a very rigid polymeric matrix based on polyvinyl alcohol (LentiKats) has…”
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Co-Aggregation of Penicillin G Acylase and Polyionic Polymers:  An Easy Methodology To Prepare Enzyme Biocatalysts Stable in Organic Media by Wilson, Lorena, Illanes, Andrés, Abián, Olga, Pessela, Benevides C. C, Fernández-Lafuente, Roberto, Guisán, José M

“…A novel type of biocatalyst that combines the good properties of cross-linked enzyme aggregates (CLEAs) and hydrophilic microenvironments has been developed…”
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Biotechnological Applications of Proteases in Food Technology by Tavano, Olga Luisa, Berenguer‐Murcia, Angel, Secundo, Francesco, Fernandez‐Lafuente, Roberto

“…This review presents some of the hottest topics in biotechnological applications: proteases in biocatalysis. Obviously, one of the most relevant areas of…”
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Design of Bactericidal Peptides Against Escherichia coli O157:H7, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus by Cruz, Jenniffer, Rondon-Villarreal, Paola, Torres, Rodrigo G, Urquiza, Mauricio, Guzman, Fanny, Alvarez, Claudio, Abengozar, Maria A, Sierra, Daniel A, Rivas, Luis, Fernandez-Lafuente, Roberto, Ortiz, Claudia C

“…Antimicrobial peptides are on the first line of defense against pathogenic microorganisms of many living beings. These compounds are considered natural…”
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Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment by ABIAN, Olga, GRAZU, Valeria, HERMOSO, Juan, GONZALEZ, Ramon, GARCIA, José Luis, FERNANDEZ-LAFUENTE, Roberto, GUISAN, José Manuel

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Stabilization of different alcohol oxidases via immobilization and post immobilization techniques by Lopez-Gallego, Fernando, Betancor, Lorena, Hidalgo, Aurelio, Dellamora-Ortiz, Gisela, Mateo, Cesar, Fernández-Lafuente, Roberto, Guisán, Jose M.

“…The thermal stability of multimeric alcohol oxidases (AOXs) from three different sources ( Candida boidinii, Hansenula sp. and Pichia pastoris) was evaluated…”
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Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β-lactam antibiotics by Romero, Oscar, Vergara, José, Fernández-Lafuente, Roberto, Guisán, José Manuel, Illanes, Andrés, Wilson, Lorena

“…Some reactions of organic synthesis require to be performed in rather aggressive media, like organic solvents, that frequently impair enzyme operational…”
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Immobilization on octyl‐agarose beads and some catalytic features of commercial preparations of lipase a from Candida antarctica (Novocor ADL): Comparison with immobilized lipase B from Candida antarctica by Arana‐Peña, Sara, Lokha, Yuliya, Fernández‐Lafuente, Roberto

“…Lipase A from Candida antarctica (CALA, commercialized as Novocor ADL) was immobilized on octyl‐agarose, which is a very useful support for lipase…”
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